Cytomegalovirus: pathophysiological mechanisms of the cytomegalovirus-induced cellular responses
Cytomegalovirus (CMV) infection of fibroblasts of human origin is associated with a cascade of morphologic cellular responses which in other systems have been associated with regulation of intracellular free (IF) (Ca/sup + +/). In the present study, the relationship of specific ion fluxes (Ca/sup + +/, Na/sup +/) to the development of cytomegalovirus (CMV)-induced morphologic cellular responses was investigated. An influx of Ca/sup + +/ was observed by the first hour after CMV infection (PI), and total calcium sequestered by infected cells was enhanced by 5 hr Pl. A gradual rise in intracellular free (IF) (Ca/sup + +/) was observed that continued through 48 hour postinfection (hr Pl). The IF (Ca/sup + +/) response to CMV infection was shown to be multiplicity dependent, require viable virus, and occur under conditions consistent with the expression of immediate early CMV genes. Development and progression of cytomegaly was found to be independent of CMV DNA synthesis and appeared to be dependent on the IF (Ca/sup + +/) response. Ca/sup + +/ influx blockers (e.g. verapamil) and cyclic nucleotide modulators (e.g. papaverine) inhibited both Ca/sup + +/ responses and cytomegaly. Quabain-sensitive /sup 86/Rb uptake and sequestering of Ca/sup + +/ increased in parallel with development of cytomegaly. There may be a relationship between Ca/sup + +/ influx, IF (Ca/sup + +/), activation of the Na/sup +//H/sup +/ exchanger, induction of Na/sup +/, Cl/sup -/, HCO/sub 3/ cotransport, Na/sup +/ entry, Na/sup +//K/sup +/ ATPase activity and development of CMV-induced morphologic cellular responses including cytomegaly.
- Research Organization:
- Texas Univ., Houston (USA). Graduate School of Biomedical Sciences
- OSTI ID:
- 6546233
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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CALCIUM COMPOUNDS
MEMBRANE TRANSPORT
RUBIDIUM 86
VIRAL DISEASES
PATHOGENESIS
CATIONS
FIBROBLASTS
SODIUM COMPOUNDS
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ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
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INTERMEDIATE MASS NUCLEI
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ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
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RUBIDIUM ISOTOPES
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