Involvement of plasma membrane calcium influx in bacterial induction of the K sup + /H sup + and hypersensitive responses in tobacco. [Pseudomonas syringae pv syringae]

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.92.1.215· OSTI ID:7192038

Atkinson, M M; Keppler, L D; Orlandi, E W; Baker, C J; Mischke, C F ^[1]

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1. Dept. of Agriculture, Beltsville, MD (USA) Univ. of Maryland, College Park (USA)

An early event in the hypersensitive response of tobacco to Pseudomonas syringae pv syringae is the initiation of a K{sup +}/H{sup +} response characterized by specific plasma membrane K{sup +} efflux, extracellular alkalinization, and intracellular acidification. We investigated the role of calcium in induction of these host responses. Suspension-cultured tobacco cells exhibited a baseline Ca{sup 2+} influx of 0.02 to 0.06 micromole per gram per hour as determined from {sup 45}Ca{sup 2+} uptake. Following bacterial inoculation, uptake rates began to increase coincidently with onset of the K{sup +}/H{sup +} response. Rates increased steadily for 2 to 3 hours, reaching 0.5 to 1 micromole per gram per hour. This increased Ca{sup 2+} influx was prevented by EGTA and calcium channel blockers such as La{sup 3+}, Co{sup 2+}, and Cd{sup 2+} but not by verapamil and nifedipine. Lanthanum, cobalt, cadmium, and EGTA inhibited the K{sup +}/H{sup +} response in both suspension-cultured cells and leaf discs and prevented hypersensitive cell death in leaf discs. We conclude that increase plasmalemma Ca{sup 2+} influx is required for the K{sup +}/H{sup +} and hypersensitive responses in tobacco.

• OSTI ID: 7192038
• Journal Information: Plant Physiology; (USA), Vol. 92:1; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM COMPOUNDS
BIOLOGICAL FUNCTIONS
PLANT CELLS
SENSITIVITY
PSEUDOMONAS
INFECTIVITY
BIOLOGICAL PATHWAYS
CALCIUM 45
CELL MEMBRANES
HYDROGEN
MEMBRANE TRANSPORT
POTASSIUM
TOBACCO
TRACER TECHNIQUES
ALKALI METALS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
FUNCTIONS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANES
METALS
MICROORGANISMS
NONMETALS
NUCLEI
RADIOISOTOPES
551001* - Physiological Systems- Tracer Techniques