Rabbit blastocysts accumulate (/sup 3/H)prostaglandins in vitro

Journal Article · Wed Aug 01 00:00:00 EDT 1984 · Endocrinology; (United States)

OSTI ID:6204368

Jones, M A; Harper, M J

Rabbit blastocysts obtained on days 5, 6, and 6.8 of pregnancy were incubated in vitro in Tyrode's buffer with /sup 3/H-labeled prostaglandins (PGs). Accumulation of PGs was studied, using Whatman GF/F filters to separate bound and free ligands. The uptake and efflux of (/sup 3/H)PGs were studied as a function of PG type, incubation time, temperature, and effect of metabolic inhibitors as well as age and number of blastocysts. Blastocysts of the same age accumulated approximately the same amount of (/sup 3/H)PGE2 and (/sup 3/H)PGF2 alpha from their environment; however, there was no apparent saturation over a PG concentration range of 1-1000 nM. Both the uptake and efflux of PG were age dependent, with older blastocysts accumulating more PGs. Approximately 90% of the (/sup 3/H)PGs appear to be transported into the blastocoelic fluid, with little PG remaining in the blastomeres. PG accumulation was relatively insensitive to azide, ouabain, cyanide, or bromcresol green, but was affected by incubation at 0 C or the addition of indomethacin (10 micrograms/ml). No catabolism of the accumulated PGs was observed. The release of PGE2 in general did not differ from that of PGF2 alpha, except on day 6.8 of pregnancy when PGE2 was released more rapidly than on day 6. The authors conclude that rabbit blastocysts can accumulate PGs from their environment, which may imply a storage potential in the blastocyst and release before implantation.

• Research Organization: Univ. of Texas Health Science Center, San Antonio
• OSTI ID: 6204368
• Journal Information: Endocrinology; (United States), Vol. 115:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EMBRYONIC CELLS
BIOCHEMISTRY
PROSTAGLANDINS
BIOCHEMICAL REACTION KINETICS
ANTIPYRETICS
CELL CULTURES
IN VITRO
RABBITS
RESPONSE MODIFYING FACTORS
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
ANIMAL CELLS
ANIMALS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMISTRY
DRUGS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
REACTION KINETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques