Prostaglandin (PG) synthesis by hepatoma cells
Proliferation of cultured HTC hepatoma cells was reported to be inhibited by indomethacin but synthesis of PG in these cells was no detected. The authors have found that omission of fetal calf serum from the medium permits detection of synthesis of 6-keto-PGF1 alpha, PFG2 alpha, PGE2 and TxB2 from labeled arachidonic acid. Two additional peaks were identified as metabolites of PGF2 alpha and PGE2 by retention times on HPLC. Indomethacin inhibited the formation of the PGs and the metabolites. When /sup 3/H-PGE2 and /sup 3/H-PGF2 alpha were added to the cultures, approximately 50% of the label was recovered as the PG metabolites after a 4 day incubation. Metabolism of /sup 3/H-TxB2 was not detected. When HTC cells were grown in the presence of 100 ..mu..M flurbiprofen, a cyclooxygenase inhibitor, there was significant inhibition of both cell proliferation and /sup 3/H-thymidine uptake. The authors data suggest that proliferation of hepatoma cells is facilitated by synthesis of PGs.
- Research Organization:
- UNDNJ-New Jersey Medical School, Newark
- OSTI ID:
- 6074829
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROSTAGLANDINS
BIOSYNTHESIS
TUMOR CELLS
CELL PROLIFERATION
ARACHIDONIC ACID
ENZYME INHIBITORS
HEPATOMAS
INHIBITION
LIQUID COLUMN CHROMATOGRAPHY
METABOLITES
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
AZINES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DISEASES
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MONOCARBOXYLIC ACIDS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SEPARATION PROCESSES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques