Evaluation of spray-drying methods for cellulase preservation

Conference · Wed Jan 01 00:00:00 EST 1986 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:5811693

Himmel, M; Oh, K; Tucker, M; Rivard, C; Grohmann, K; Scott, C D ^[1]

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1. ed.

Spray drying processes are widely used for the large-scale preservation of biology goods. The application of this technology to the enhancement of the storability of cellulase and related enzymes would improve fermentation processes requiring input of specific quantities of enzyme catalyst of known activity. Very high percentages (90% of original) of filter paper and carboxymethyicellulose hydrolyzing activities can be recovered from conventional spray dryers when operated at inlet air temperatures ranging from 90 to 180 degrees. The beta-glucosidase and beta-xylosidase activity recoveries were somewhat lower and were optimal at temperatures ranging from 120 to 150 degrees. However, the individual performance of the 12 enzyme preparations examined was quite preparation specific. An ultrasonic nozzle adaptation to the Yamato dryer demonstrated the overall feasibility of high energy atomization inasmuch as the filter paper activity was retained after drying. The more labile beta-xylosidase activity was, however, entirely lost.

• Research Organization: Solar Energy Research Institute, Golden, CO 80401, USA. Solar Fuels Research Div.
• OSTI ID: 5811693
• Report Number(s): CONF-860508-
• Journal Information: Biotechnol. Bioeng. Symp.; (United States), Vol. 17; Conference: 8. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN, USA, 13 May 1986
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
CELLULASE
PRESERVATION
SPRAY DRYING
AIR
ATOMIZATION
BIOLOGICAL MATERIALS
DRYERS
ENERGY
ENZYME ACTIVITY
EVALUATION
FEASIBILITY STUDIES
FILTERS
MEDIUM TEMPERATURE
OPERATION
OPTIMIZATION
PAPER
PERFORMANCE
RECOVERY
STORAGE
TECHNOLOGY UTILIZATION
DRYING
ENZYMES
FLUIDS
GASES
GLYCOSYL HYDROLASES
HYDROLASES
MATERIALS
O-GLYCOSYL HYDROLASES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)