Kinetics of growth and differentiation of cultured human epidermal keratinocytes
A study was made of the interrelationship between replication and differentiation in cultures of human epidermal keratinocytes. Measures of both parameters were made using newly developed methods to quantify the rate at which keratinocytes replicate and the rate at which they withdraw from the cell cycle. Keratinocyte replication was measured by determining the cell doubling time, labeling index, and cell cycle duration. Cell cycle length was measured using a double label assay that determines the length of time between two successive phases of DNA synthesis. The first DNA synthesis phase was marked by labeling keratinocytes with /sup 14/C-thymidine. At the next round of DNA synthesis, cells were labeled with bromodeoxyuridine, a heavy analog of thymidine. The cell cycle length is given by the time required for the /sup 14/C-labeled DNA to become double labeled. To measure keratinocyte differentiation, the rate at which cells withdraw from the cell cycle was determined. To measure withdrawal, the percentage of cells labeled by a pulse of /sup 14/C-thymidine that failed to undergo a second cycle of DNA synthesis, as measured by bromodeoxyuridine incorporation, was determined. Cells which failed to undergo a second cycle of synthesis were considered to have differentiated and withdrawn from the cell cycle.
- Research Organization:
- State Univ. of New York, Stony Brook (USA)
- OSTI ID:
- 5681622
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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EPIDERMIS
CELL DIFFERENTIATION
DNA REPLICATION
BIOCHEMICAL REACTION KINETICS
BUDR
CARBON 14 COMPOUNDS
CELL CULTURES
CELL CYCLE
DNA
GROWTH
KERATIN
MAN
THYMIDINE
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
ANTIMETABOLITES
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BODY
BROMOURACILS
DRUGS
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HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
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MAMMALS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PRIMATES
PROTEINS
PYRIMIDINES
REACTION KINETICS
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550301* - Cytology- Tracer Techniques