Macrophages do not inhibit the participation of the nuclei of nonmalignant proliferating cells in DNA synthesis in heterokaryons

Journal Article · Mon Jul 01 00:00:00 EDT 1985 · Dokl. Biol. Sci. (Engl. Transl.); (United States)

OSTI ID:5841831

Egorov, E E; Prudovskii, I A; Zelenin, A V

The authors continue their investigations into types of heterokaryons in an effort to detect an inhibition of nondividing macrophages (differentiated cells) on the entry of the nuclei of proliferating cells into replication. For the experiments described in this paper, the authors used asynchronous cultures of mouse diploid fibroblasts (MDF), 3T3 mouse cells from continuous culture, and malignant SV3T3 cells (3T3 cells transformed by SV40). Fusion of the cells of the cultures with macrophages was performed using PEG at various periods after deposition (2, 8, 12, and 20 h). The authors used double isotope marking to identify DNA synthesis in the heterokaryons. For this purpose, the nuclei of the culture cells were labeled with (/sup 3/H)thymidine before fusion with macrophages. All the nuclei of the culture cells intensively incorporated the label. After fusion, (/sup 14/C)thymidine was introduced into the incubation medium. If the cell nucleus began to synthesize DNA, it incorporated (/sup 14/C)thymidine, and a supplementary relatively weak label appeared on the auto-radiographic preparations, both above the nuclei themselves and next to them. The nuclei of macrophages in which DNA synthesis was reactivated contained only the (/sup 14/C)label. Fixation was performed 26 h after stimulation (in the case of 3T3) or 35 h after stimulation (for MDF). The percentages of nuclei of culture cells labeled with (/sup 14/C)thymidine were determined in the heterokaryons and free-lying cells.

• Research Organization: Institute of Molecular Biology, Moscow, USSR
• OSTI ID: 5841831
• Journal Information: Dokl. Biol. Sci. (Engl. Transl.); (United States), Vol. 280:1-6; Other Information: Translated from Dokl. Akad. Nauk SSSR; 280: No. 4, 1016-1019(Feb 1985)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL NUCLEI
DNA REPLICATION
TRACER TECHNIQUES
DNA
BIOSYNTHESIS
MACROPHAGES
BIOLOGICAL EFFECTS
BIOCHEMISTRY
CARBON 14 COMPOUNDS
CELL CULTURES
CELL PROLIFERATION
DOUBLE LABELLING
FIBROBLASTS
INHIBITION
MICE
RADIONUCLIDE KINETICS
THYMIDINE
TUMOR CELLS
UPTAKE
ANIMAL CELLS
ANIMALS
AZINES
CELL CONSTITUENTS
CHEMISTRY
CONNECTIVE TISSUE CELLS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAMMALS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHAGOCYTES
PYRIMIDINES
RIBOSIDES
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550301* - Cytology- Tracer Techniques
550401 - Genetics- Tracer Techniques