Action of 50 Hz magnetic fields on cyclic AMP and intercellular communication in monolayers and spheroids of mammalian cells
- Research Center Karlsruhe (Germany). Inst. of Toxicology
To investigate the influence of physiological parameters such as cell density and three-dimensional cell contact on the biological action of a 2mT/50 Hz magnetic field, mouse fibroblasts were exposed as monolayers and as multicellular spheroids. Changes in cyclic AMP content of cells and alterations in gap junction-mediated intercellular communication were measured immediately after 5 min of exposure to the field. In monolayers of intermediate cell density (1 {times} 10{sup 5} cells/cm{sup 2}), the field treatment caused an increase in cAMP to 121% of the control level, whereas, at 3 {times} 10{sup 5} cells/cm{sup 2} (near confluence), a decrease to 88% of the unexposed cells was observed. Furthermore, field exposure stimulated gap-junction communication to 160% of the control level as determined by Lucifer yellow dye exchange. In spheroids, alterations in the radial profile of cellular cAMP were observed that were due both to field-induced local cAMP changes and to increased gap-junction permeability for this second messenger, the latter causing radial cAMP gradients to be flattened. The results indicate a strong dependence of field action on physiological parameters of the system exposed.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 201124
- Journal Information:
- Bioelectromagnetics, Vol. 16, Issue 6; Other Information: PBD: 1995
- Country of Publication:
- United States
- Language:
- English
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