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Title: Simultaneous Production of Triacylglycerol and High-Value Carotenoids by the Astaxanthin-Producing Oleaginous Green Microalga Chlorella zofingiensis

The production of lipids and astaxanthin, a high-value carotenoid, by Chlorella zofingiensis was investigated under different culture conditions. Comparative analysis revealed a good correlation between triacylglycerol (TAG) and astaxanthin accumulation in C. zofingiensis. Stress conditions promoted cell size and weight and induced the accumulation of neutral lipids, especially TAG and astaxanthin, with a concomitant decrease in membrane lipids. The highest contents of TAG and astaxanthin achieved were 387 and 4.89 mg g-1 dry weight, respectively. A semi-continuous culture strategy was developed to optimize the TAG and astaxanthin productivities, which reached 297 and 3.3 mg L-1 day-1, respectively. Additionally, astaxanthin accumulation was enhanced by inhibiting de novo fatty acid biosynthesis. In summary, our study represents a pioneering work of utilizing Chlorella for the integrated production of lipids and high-value products and C. zofingiensis has great potential to be a promising production strain and serve as an emerging oleaginous model alga.
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Publication Date:
OSTI Identifier:
Report Number(s):
Journal ID: ISSN 0960-8524
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Bioresource Technology; Journal Volume: 214; Related Information: Bioresource Technology
Research Org:
NREL (National Renewable Energy Laboratory (NREL), Golden, CO (United States))
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Country of Publication:
United States
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY astaxanthin; biofuels; Chlorella zofingiensis; integrated production; stress conditions; triacylglycerol