Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens

Liu, Sheng; Huh, Hyun; Lee, Sang-Hyuk; Huang, Fang

doi:10.1146/annurev-bioeng-060418-052203

Title: Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens

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Abstract

Super-resolution microscopy techniques are versatile and powerful tools for visualizing organelle structures, interactions, and protein functions in biomedical research. However, whole-cell and tissue specimens challenge the achievable resolution and depth of nanoscopy methods. We focus on three-dimensional single-molecule localization microscopy and review some of the major roadblocks and developing solutions to resolving thick volumes of cells and tissues at the nanoscale in three dimensions. These challenges include background fluorescence, system- and sample-induced aberrations, and information carried by photons, as well as drift correction, volume reconstruction, and photobleaching mitigation. We also highlight examples of innovations that have demonstrated significant breakthroughs in addressing the abovementioned challenges together with their core concepts as well as their trade-offs.

Authors:

Liu, Sheng ^[1]; Huh, Hyun ^[2]; Lee, Sang-Hyuk ^[3]; Huang, Fang ^[4]

Purdue Univ., West Lafayette, IN (United States). Weldon School of Biomedical Engineering
Rutgers Univ., Piscataway, NJ (United States). Inst. for Quantitative Biomedicine
Rutgers Univ., Piscataway, NJ (United States). Inst. for Quantitative Biomedicine. Dept. of Physics and Astronomy
Purdue Univ., West Lafayette, IN (United States). Weldon School of Biomedical Engineering. Purdue Inst. for Integrative Neuroscience. Purdue Inst. of Inflammation, Immunology, and Infectious Disease

Publication Date:: Fri Apr 03 00:00:00 EDT 2020

Research Org.:: Rutgers Univ., Piscataway, NJ (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

OSTI Identifier:: 1803680

Alternate Identifier(s):: OSTI ID: 1881180

Grant/Contract Number:: SC0019313

Resource Type:: Accepted Manuscript

Journal Name:: Annual Review of Biomedical Engineering

Additional Journal Information:: Journal Volume: 22; Journal Issue: 1; Journal ID: ISSN 1523-9829

Publisher:: Annual Reviews

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; super-resolution microscopy; fluorescence microscopy; tissue imaging; adaptive optics; light-sheet microscopy; Cramér–Rao lower bound; CRLB

Citation Formats


                    Liu, Sheng, Huh, Hyun, Lee, Sang-Hyuk, and Huang, Fang. Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens.  United States: N. p., 2020. 
Web.  doi:10.1146/annurev-bioeng-060418-052203.

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                    Liu, Sheng, Huh, Hyun, Lee, Sang-Hyuk, & Huang, Fang. Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens.  United States.  https://doi.org/10.1146/annurev-bioeng-060418-052203

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                    Liu, Sheng, Huh, Hyun, Lee, Sang-Hyuk, and Huang, Fang. Fri .  
"Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens".  United States.  https://doi.org/10.1146/annurev-bioeng-060418-052203.  https://www.osti.gov/servlets/purl/1803680.

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@article{osti_1803680,

  title        = {Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens},

  author       = {Liu, Sheng and Huh, Hyun and Lee, Sang-Hyuk and Huang, Fang},

  abstractNote = {Super-resolution microscopy techniques are versatile and powerful tools for visualizing organelle structures, interactions, and protein functions in biomedical research. However, whole-cell and tissue specimens challenge the achievable resolution and depth of nanoscopy methods. We focus on three-dimensional single-molecule localization microscopy and review some of the major roadblocks and developing solutions to resolving thick volumes of cells and tissues at the nanoscale in three dimensions. These challenges include background fluorescence, system- and sample-induced aberrations, and information carried by photons, as well as drift correction, volume reconstruction, and photobleaching mitigation. We also highlight examples of innovations that have demonstrated significant breakthroughs in addressing the abovementioned challenges together with their core concepts as well as their trade-offs.},

  doi          = {10.1146/annurev-bioeng-060418-052203},

  journal      = {Annual Review of Biomedical Engineering},

  number       = 1,

  volume       = 22,

  place        = {United States},

  year         = {Fri Apr 03 00:00:00 EDT 2020},

  month        = {Fri Apr 03 00:00:00 EDT 2020}

}

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