Identification of PADI2 as a potential breast cancer biomarker and therapeutic target
Abstract
Background: We have recently reported that the expression of peptidylarginine deiminase 2 (PADI2) is regulated by EGF in mammary cancer cells and appears to play a role in the proliferation of normal mammary epithelium; however, the role of PADI2 in the pathogenesis of human breast cancer has yet to be investigated. Thus, the goals of this study were to examine whether PADI2 plays a role in mammary tumor progression, and whether the inhibition of PADI activity has anti-tumor effects. Methods: RNA-seq data from a collection of 57 breast cancer cell lines was queried for PADI2 levels, and correlations with known subtype and HER2/ERBB2 status were evaluated. To examine PADI2 expression levels during breast cancer progression, the cell lines from the MCF10AT model were used. The efficacy of the PADI inhibitor, Cl-amidine, was tested in vitro using MCF10DCIS cells grown in 2D-monolayers and 3D-spheroids, and in vivo using MCF10DCIS tumor xenografts. Treated MCF10DCIS cells were examined by flow-cytometry to determine the extent of apoptosis and by RT2 Profiler PCR Cell Cycle Array to detect alterations in cell cycle associated genes. Results: We show by RNA-seq that PADI2 mRNA expression is highly correlated with HER2/ERBB2 (p = 2.2 × 106 ) inmore »
- Authors:
-
- Cornell Univ., Ithaca, NY (United States). College of Veterinary Medicine. Baker Inst. for Animal Health
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division
- Univ. of Wyoming, Laramie, WY (United States). Dept. of Zoology and Physiology
- Cornell Univ., Ithaca, NY (United States). Weill Medical College. Dept. of Cell and Developmental Biology
- Scripps Research Inst., Jupiter, FL (United States). Dept. of Chemistry
- Univ. of South Carolina, Columbia, SC (United States). Dept. of Chemistry and Biochemistry
- Oregon Health and Science Univ., Portland, OR (United States). Dept. of Biomedical Engineering
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
- OSTI Identifier:
- 1626525
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- BMC Cancer
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 1; Journal ID: ISSN 1471-2407
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Oncology; Peptidylarginine deiminase; PAD2/PADI2; HER2/ERBB2; Breast cancer; Luminal; Cl-amidine; Citrullination
Citation Formats
McElwee, John L., Mohanan, Sunish, Griffith, Obi L., Breuer, Heike C., Anguish, Lynne J., Cherrington, Brian D., Palmer, Ashley M., Howe, Louise R., Subramanian, Venkataraman, Causey, Corey P., Thompson, Paul R., Gray, Joe W., and Coonrod, Scott A. Identification of PADI2 as a potential breast cancer biomarker and therapeutic target. United States: N. p., 2012.
Web. doi:10.1186/1471-2407-12-500.
McElwee, John L., Mohanan, Sunish, Griffith, Obi L., Breuer, Heike C., Anguish, Lynne J., Cherrington, Brian D., Palmer, Ashley M., Howe, Louise R., Subramanian, Venkataraman, Causey, Corey P., Thompson, Paul R., Gray, Joe W., & Coonrod, Scott A. Identification of PADI2 as a potential breast cancer biomarker and therapeutic target. United States. https://doi.org/10.1186/1471-2407-12-500
McElwee, John L., Mohanan, Sunish, Griffith, Obi L., Breuer, Heike C., Anguish, Lynne J., Cherrington, Brian D., Palmer, Ashley M., Howe, Louise R., Subramanian, Venkataraman, Causey, Corey P., Thompson, Paul R., Gray, Joe W., and Coonrod, Scott A. Tue .
"Identification of PADI2 as a potential breast cancer biomarker and therapeutic target". United States. https://doi.org/10.1186/1471-2407-12-500. https://www.osti.gov/servlets/purl/1626525.
@article{osti_1626525,
title = {Identification of PADI2 as a potential breast cancer biomarker and therapeutic target},
author = {McElwee, John L. and Mohanan, Sunish and Griffith, Obi L. and Breuer, Heike C. and Anguish, Lynne J. and Cherrington, Brian D. and Palmer, Ashley M. and Howe, Louise R. and Subramanian, Venkataraman and Causey, Corey P. and Thompson, Paul R. and Gray, Joe W. and Coonrod, Scott A.},
abstractNote = {Background: We have recently reported that the expression of peptidylarginine deiminase 2 (PADI2) is regulated by EGF in mammary cancer cells and appears to play a role in the proliferation of normal mammary epithelium; however, the role of PADI2 in the pathogenesis of human breast cancer has yet to be investigated. Thus, the goals of this study were to examine whether PADI2 plays a role in mammary tumor progression, and whether the inhibition of PADI activity has anti-tumor effects. Methods: RNA-seq data from a collection of 57 breast cancer cell lines was queried for PADI2 levels, and correlations with known subtype and HER2/ERBB2 status were evaluated. To examine PADI2 expression levels during breast cancer progression, the cell lines from the MCF10AT model were used. The efficacy of the PADI inhibitor, Cl-amidine, was tested in vitro using MCF10DCIS cells grown in 2D-monolayers and 3D-spheroids, and in vivo using MCF10DCIS tumor xenografts. Treated MCF10DCIS cells were examined by flow-cytometry to determine the extent of apoptosis and by RT2 Profiler PCR Cell Cycle Array to detect alterations in cell cycle associated genes. Results: We show by RNA-seq that PADI2 mRNA expression is highly correlated with HER2/ERBB2 (p = 2.2 × 106 ) in luminal breast cancer cell lines. Using the MCF10AT model of breast cancer progression, we then demonstrate that PADI2 expression increases during the transition of normal mammary epithelium to fully malignant breast carcinomas, with a strong peak of PADI2 expression and activity being observed in the MCF10DCIS cell line, which models human comedo-DCIS lesions. Next, we show that a PADI inhibitor, Cl-amidine, strongly suppresses the growth of MCF10DCIS monolayers and tumor spheroids in culture. We then carried out preclinical studies in nude (nu/nu) mice and found that Cl-amidine also suppressed the growth of xenografted MCF10DCIS tumors by more than 3-fold. Lastly, we performed cell cycle array analysis of Cl-amidine treated and control MCF10DCIS cells, and found that the PADI inhibitor strongly affects the expression of several cell cycle genes implicated in tumor progression, including p21, GADD45α, and Ki67. Conclusion: Together, these results suggest that PADI2 may function as an important new biomarker for HER2/ERBB2+ tumors and that Cl-amidine represents a new candidate for breast cancer therapy.},
doi = {10.1186/1471-2407-12-500},
journal = {BMC Cancer},
number = 1,
volume = 12,
place = {United States},
year = {Tue Oct 30 00:00:00 EDT 2012},
month = {Tue Oct 30 00:00:00 EDT 2012}
}
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Potential Role for Peptidylarginine Deiminase 2 (PAD2) in Citrullination of Canine Mammary Epithelial Cell Histones
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Comedo-Ductal carcinoma in situ: A paradoxical role for programmed cell death
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Cytoplasmic accumulation of the RNA binding protein HuR is central to tamoxifen resistance in estrogen receptor positive breast cancer cells
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