H2-Independent Growth of the Hydrogenotrophic Methanogen Methanococcus maripaludis
Abstract
Hydrogenotrophic methanogenic Archaea require reduced ferredoxin as an anaplerotic source of electrons for methanogenesis. H2 oxidation by the hydrogenase Eha provides these electrons, consistent with an H2 requirement for growth. Here we report the identification of alternative pathways of ferredoxin reduction in Methanococcus maripaludis that operate independently of Eha to stimulate methanogenesis. A suppressor mutation that increased expression of the glycolytic enzyme glyceraldehyde-3-phosphate:ferredoxin oxidoreductase resulted in a strain capable of H2-independent ferredoxin reduction and growth with formate as the sole electron donor. In this background, it was possible to eliminate all seven hydrogenases of M. maripaludis. Alternatively, carbon monoxide oxidation by carbon monoxide dehydrogenase could also generate reduced ferredoxin that feeds into methanogenesis. In either case, the reduced ferredoxin generated was inefficient at stimulating methanogenesis, resulting in a slow growth phenotype. As methanogenesis is limited by the availability of reduced ferredoxin under these conditions, other electron donors, such as reduced coenzyme F420, should be abundant. Indeed, when F420-reducing hydrogenase was reintroduced into the hydrogenase-free mutant, the equilibrium of H2 production via an F420-dependent formate:H2 lyase activity shifted markedly toward H2 compared to the wild type.
- Authors:
-
- Univ. of Washington, Seattle, WA (United States). Dept. of Microbiology
- Publication Date:
- Research Org.:
- Univ. of Washington, Seattle, WA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1626081
- Grant/Contract Number:
- FG02-05ER15709
- Resource Type:
- Accepted Manuscript
- Journal Name:
- mBio (Online)
- Additional Journal Information:
- Journal Name: mBio (Online); Journal Volume: 4; Journal Issue: 2; Journal ID: ISSN 2150-7511
- Publisher:
- American Society for Microbiology (ASM)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Microbiology
Citation Formats
Costa, Kyle C., Lie, Thomas J., Jacobs, Michael A., and Leigh, John A. H2-Independent Growth of the Hydrogenotrophic Methanogen Methanococcus maripaludis. United States: N. p., 2013.
Web. doi:10.1128/mbio.00062-13.
Costa, Kyle C., Lie, Thomas J., Jacobs, Michael A., & Leigh, John A. H2-Independent Growth of the Hydrogenotrophic Methanogen Methanococcus maripaludis. United States. https://doi.org/10.1128/mbio.00062-13
Costa, Kyle C., Lie, Thomas J., Jacobs, Michael A., and Leigh, John A. Tue .
"H2-Independent Growth of the Hydrogenotrophic Methanogen Methanococcus maripaludis". United States. https://doi.org/10.1128/mbio.00062-13. https://www.osti.gov/servlets/purl/1626081.
@article{osti_1626081,
title = {H2-Independent Growth of the Hydrogenotrophic Methanogen Methanococcus maripaludis},
author = {Costa, Kyle C. and Lie, Thomas J. and Jacobs, Michael A. and Leigh, John A.},
abstractNote = {Hydrogenotrophic methanogenic Archaea require reduced ferredoxin as an anaplerotic source of electrons for methanogenesis. H2 oxidation by the hydrogenase Eha provides these electrons, consistent with an H2 requirement for growth. Here we report the identification of alternative pathways of ferredoxin reduction in Methanococcus maripaludis that operate independently of Eha to stimulate methanogenesis. A suppressor mutation that increased expression of the glycolytic enzyme glyceraldehyde-3-phosphate:ferredoxin oxidoreductase resulted in a strain capable of H2-independent ferredoxin reduction and growth with formate as the sole electron donor. In this background, it was possible to eliminate all seven hydrogenases of M. maripaludis. Alternatively, carbon monoxide oxidation by carbon monoxide dehydrogenase could also generate reduced ferredoxin that feeds into methanogenesis. In either case, the reduced ferredoxin generated was inefficient at stimulating methanogenesis, resulting in a slow growth phenotype. As methanogenesis is limited by the availability of reduced ferredoxin under these conditions, other electron donors, such as reduced coenzyme F420, should be abundant. Indeed, when F420-reducing hydrogenase was reintroduced into the hydrogenase-free mutant, the equilibrium of H2 production via an F420-dependent formate:H2 lyase activity shifted markedly toward H2 compared to the wild type.},
doi = {10.1128/mbio.00062-13},
journal = {mBio (Online)},
number = 2,
volume = 4,
place = {United States},
year = {Tue Feb 26 00:00:00 EST 2013},
month = {Tue Feb 26 00:00:00 EST 2013}
}
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