Determining the Reliability of Measuring Mercury Cycling Gene Abundance with Correlations with Mercury and Methylmercury Concentrations
Abstract
Methylmercury (MeHg) is a bioaccumulative toxic contaminant in many ecosystems, but factors governing its production are poorly understood. Recent work has shown that the anaerobic microbial conversion of mercury (Hg) to MeHg requires the Hg-methylation genes hgcAB and that these genes can be used as biomarkers in PCR-based estimators of Hg-methylator abundance. In an effort to determine reliable methods for assessing hgcA abundance and diversity and linking them to MeHg concentrations, multiple approaches were compared including metagenomic shotgun sequencing, 16S rRNA gene pyrosequencing and cloning/sequencing hgcAB gene products. Hg-methylator abundance was also determined by quantitative hgcA qPCR amplification and metaproteomics for comparison to the above measurements. Samples from eight sites were examined covering a range of total Hg (HgT; 0.03–14 mg kg–1 dry wt. soil) and MeHg (0.05–27 μg kg–1 dry wt. soil) concentrations. In the metagenome and amplicon sequencing of hgcAB diversity, the Deltaproteobacteria were the dominant Hg-methylators while Firmicutes and methanogenic Archaea were typically ~50% less abundant. This was consistent with metaproteomics estimates where the Deltaproteobacteria were steadily higher. The 16S rRNA gene pyrosequencing did not have sufficient resolution to identify hgcAB+ species. Metagenomic and hgcAB results were similar for Hg-methylator diversity and clade-specific qPCR-based approaches for hgcAmore »
- Authors:
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[1];
[1];
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- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
- Univ. of Idaho, Moscow, ID (United States). College of Engineering
- Ramapo College of New Jersey, Mahwah, NJ (United States). School of Theoretical and Applied Science
- Texas A&M Univ., Overton, TC (United States). Dept. of Soil and Crop Sciences
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division
- Montana State Univ., Bozeman, MT (United States). Center for Biofilm Engineering
- Montana State Univ., Bozeman, MT (United States). Center for Biofilm Engineering and Dept. of Microbiology and Immunology
- Univ. of Missouri, Columbia, MO (United States). Dept.of Biochemistry,
- Smithsonian Environmental Research Center, Edgewater, Maryland 21037, United States
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1557487
- Alternate Identifier(s):
- OSTI ID: 1671432
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science and Technology
- Additional Journal Information:
- Journal Volume: 53; Journal Issue: 15; Journal ID: ISSN 0013-936X
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; microbial community; PCR; qPCR; metagenome; hgcAB; 16S sequencing
Citation Formats
Christensen, Geoff A., Gionfriddo, Caitlin M., King, Andrew J., Moberly, James G., Miller, Carrie L., Somenahally, Anil C., Callister, Stephen J., Brewer, Heather, Podar, Mircea, Brown, Steven D., Palumbo, Anthony V., Brandt, Craig C., Wymore, Ann M., Brooks, Scott C., Hwang, Chiachi, Fields, Matthew W., Wall, Judy D., Gilmour, Cynthia C., and Elias, Dwayne A. Determining the Reliability of Measuring Mercury Cycling Gene Abundance with Correlations with Mercury and Methylmercury Concentrations. United States: N. p., 2019.
Web. doi:10.1021/acs.est.8b06389.
Christensen, Geoff A., Gionfriddo, Caitlin M., King, Andrew J., Moberly, James G., Miller, Carrie L., Somenahally, Anil C., Callister, Stephen J., Brewer, Heather, Podar, Mircea, Brown, Steven D., Palumbo, Anthony V., Brandt, Craig C., Wymore, Ann M., Brooks, Scott C., Hwang, Chiachi, Fields, Matthew W., Wall, Judy D., Gilmour, Cynthia C., & Elias, Dwayne A. Determining the Reliability of Measuring Mercury Cycling Gene Abundance with Correlations with Mercury and Methylmercury Concentrations. United States. https://doi.org/10.1021/acs.est.8b06389
Christensen, Geoff A., Gionfriddo, Caitlin M., King, Andrew J., Moberly, James G., Miller, Carrie L., Somenahally, Anil C., Callister, Stephen J., Brewer, Heather, Podar, Mircea, Brown, Steven D., Palumbo, Anthony V., Brandt, Craig C., Wymore, Ann M., Brooks, Scott C., Hwang, Chiachi, Fields, Matthew W., Wall, Judy D., Gilmour, Cynthia C., and Elias, Dwayne A. Mon .
"Determining the Reliability of Measuring Mercury Cycling Gene Abundance with Correlations with Mercury and Methylmercury Concentrations". United States. https://doi.org/10.1021/acs.est.8b06389. https://www.osti.gov/servlets/purl/1557487.
@article{osti_1557487,
title = {Determining the Reliability of Measuring Mercury Cycling Gene Abundance with Correlations with Mercury and Methylmercury Concentrations},
author = {Christensen, Geoff A. and Gionfriddo, Caitlin M. and King, Andrew J. and Moberly, James G. and Miller, Carrie L. and Somenahally, Anil C. and Callister, Stephen J. and Brewer, Heather and Podar, Mircea and Brown, Steven D. and Palumbo, Anthony V. and Brandt, Craig C. and Wymore, Ann M. and Brooks, Scott C. and Hwang, Chiachi and Fields, Matthew W. and Wall, Judy D. and Gilmour, Cynthia C. and Elias, Dwayne A.},
abstractNote = {Methylmercury (MeHg) is a bioaccumulative toxic contaminant in many ecosystems, but factors governing its production are poorly understood. Recent work has shown that the anaerobic microbial conversion of mercury (Hg) to MeHg requires the Hg-methylation genes hgcAB and that these genes can be used as biomarkers in PCR-based estimators of Hg-methylator abundance. In an effort to determine reliable methods for assessing hgcA abundance and diversity and linking them to MeHg concentrations, multiple approaches were compared including metagenomic shotgun sequencing, 16S rRNA gene pyrosequencing and cloning/sequencing hgcAB gene products. Hg-methylator abundance was also determined by quantitative hgcA qPCR amplification and metaproteomics for comparison to the above measurements. Samples from eight sites were examined covering a range of total Hg (HgT; 0.03–14 mg kg–1 dry wt. soil) and MeHg (0.05–27 μg kg–1 dry wt. soil) concentrations. In the metagenome and amplicon sequencing of hgcAB diversity, the Deltaproteobacteria were the dominant Hg-methylators while Firmicutes and methanogenic Archaea were typically ~50% less abundant. This was consistent with metaproteomics estimates where the Deltaproteobacteria were steadily higher. The 16S rRNA gene pyrosequencing did not have sufficient resolution to identify hgcAB+ species. Metagenomic and hgcAB results were similar for Hg-methylator diversity and clade-specific qPCR-based approaches for hgcA are only appropriate when comparing the abundance of a particular clade across various samples. Weak correlations between Hg-methylating bacteria and soil Hg concentrations were observed for similar environmental samples, but overall total Hg and MeHg concentrations poorly correlated with Hg-cycling genes.},
doi = {10.1021/acs.est.8b06389},
journal = {Environmental Science and Technology},
number = 15,
volume = 53,
place = {United States},
year = {Mon Jul 01 00:00:00 EDT 2019},
month = {Mon Jul 01 00:00:00 EDT 2019}
}
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Figures / Tables:
Table 1: Site Hg Concentrations and hgcA(B) Diversity and Abundance by Methods
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Works referenced in this record:
Mercury-methylating genes dsrB and hgcA in soils/sediments of the Three Gorges Reservoir
journal, December 2016
- Du, Hongxia; Ma, Ming; Sun, Tao
- Environmental Science and Pollution Research, Vol. 24, Issue 5
Development and Validation of Broad-Range Qualitative and Clade-Specific Quantitative Molecular Probes for Assessing Mercury Methylation in the Environment
journal, July 2016
- Christensen, Geoff A.; Wymore, Ann M.; King, Andrew J.
- Applied and Environmental Microbiology, Vol. 82, Issue 19
Stoichiometry and temperature sensitivity of methanogenesis and CO 2 production from saturated polygonal tundra in Barrow, Alaska
journal, November 2014
- Roy Chowdhury, Taniya; Herndon, Elizabeth M.; Phelps, Tommy J.
- Global Change Biology, Vol. 21, Issue 2
Challenges and opportunities for managing aquatic mercury pollution in altered landscapes
journal, January 2018
- Hsu-Kim, Heileen; Eckley, Chris S.; Achá, Dario
- Ambio, Vol. 47, Issue 2
The English–Wabigoon River System: I. A Synthesis of Recent Research with a View towards Mercury Amelioration
journal, December 1983
- Rudd, John W. M.; Turner, Michael A.; Furutani, Akira
- Canadian Journal of Fisheries and Aquatic Sciences, Vol. 40, Issue 12
Molecular composition of organic matter controls methylmercury formation in boreal lakes
journal, February 2017
- Bravo, Andrea G.; Bouchet, Sylvain; Tolu, Julie
- Nature Communications, Vol. 8, Issue 1
Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
journal, July 2006
- DeSantis, T. Z.; Hugenholtz, P.; Larsen, N.
- Applied and Environmental Microbiology, Vol. 72, Issue 7, p. 5069-5072
nhmmer: DNA homology search with profile HMMs
journal, July 2013
- Wheeler, T. J.; Eddy, S. R.
- Bioinformatics, Vol. 29, Issue 19
Mercury methylating microbial communities of boreal forest soils
journal, January 2019
- Xu, Jingying; Buck, Moritz; Eklöf, Karin
- Scientific Reports, Vol. 9, Issue 1
Syntrophs Dominate Sequences Associated with the Mercury Methylation-Related Gene hgcA in the Water Conservation Areas of the Florida Everglades
journal, August 2014
- Bae, Hee-Sung; Dierberg, Forrest E.; Ogram, Andrew
- Applied and Environmental Microbiology, Vol. 80, Issue 20
Quantification of Mercury Bioavailability for Methylation Using Diffusive Gradient in Thin-Film Samplers
journal, June 2018
- Ndu, Udonna; Christensen, Geoff A.; Rivera, Nelson A.
- Environmental Science & Technology, Vol. 52, Issue 15
Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths
journal, September 2008
- Hwang, Chiachi; Wu, Weimin; Gentry, Terry J.
- The ISME Journal, Vol. 3, Issue 1
Methyl-Mercury Degradation Pathways: A Comparison among Three Mercury-Impacted Ecosystems
journal, December 2000
- Marvin-DiPasquale, Mark; Agee, Jennifer; McGowan, Chad
- Environmental Science & Technology, Vol. 34, Issue 23
Quantitative Proteomic Analysis of Biological Processes and Responses of the Bacterium Desulfovibrio desulfuricans ND132 upon Deletion of Its Mercury Methylation Genes
journal, August 2018
- Qian, Chen; Chen, Hongmei; Johs, Alexander
- PROTEOMICS, Vol. 18, Issue 17
Sequencing Intractable DNA to Close Microbial Genomes
journal, July 2012
- Hurt, Richard A.; Brown, Steven D.; Podar, Mircea
- PLoS ONE, Vol. 7, Issue 7, Article No. e41295
Review and re-analysis of domain-specific 16S primers
journal, December 2003
- Baker, G. C.; Smith, J. J.; Cowan, D. A.
- Journal of Microbiological Methods, Vol. 55, Issue 3
Potential Hg methylation and MeHg demethylation rates related to the nutrient status of different boreal wetlands
journal, April 2011
- Tjerngren, I.; Karlsson, T.; Björn, E.
- Biogeochemistry, Vol. 108, Issue 1-3
Toward Accurate and Quantitative Comparative Metagenomics
journal, August 2016
- Nayfach, Stephen; Pollard, Katherine S.
- Cell, Vol. 166, Issue 5
Mercury Methylation by Novel Microorganisms from New Environments
journal, September 2013
- Gilmour, Cynthia C.; Podar, Mircea; Bullock, Allyson L.
- Environmental Science & Technology, Vol. 47, Issue 20
Production and Loss of Methylmercury and Loss of Total Mercury from Boreal Forest Catchments Containing Different Types of Wetlands †
journal, January 1996
- St. Louis, Vincent L.; Rudd, John W. M.; Kelly, Carol A.
- Environmental Science & Technology, Vol. 30, Issue 9
Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy
journal, June 2007
- Wang, Q.; Garrity, G. M.; Tiedje, J. M.
- Applied and Environmental Microbiology, Vol. 73, Issue 16
Water Column Methylmercury in the Wabigoon/English River–Lake System: Factors Controlling Concentrations, Speciation, and Net Production
journal, December 1989
- Parks, J. W.; Lutz, A.; Sutton, J. A.
- Canadian Journal of Fisheries and Aquatic Sciences, Vol. 46, Issue 12
Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury Methylation
journal, April 2011
- Gilmour, Cynthia C.; Elias, Dwayne A.; Kucken, Amy M.
- Applied and Environmental Microbiology, Vol. 77, Issue 12, p. 3938-3951
Degradation of methyl and ethyl mercury by singlet oxygen generated from sea water exposed to sunlight or ultraviolet light
journal, June 1993
- Suda, Ikuo; Suda, Mari; Hirayama, Kimiko
- Archives of Toxicology, Vol. 67, Issue 5
Hidden Markov model speed heuristic and iterative HMM search procedure
journal, August 2010
- Johnson, L. Steven; Eddy, Sean R.; Portugaly, Elon
- BMC Bioinformatics, Vol. 11, Issue 1
Distribution of Total Mercury and Methyl Mercury in Water, Sediment, and Fish from South Florida Estuaries
journal, February 1998
- Kannan, K.; Jr., R. G. Smith; Lee, R. F.
- Archives of Environmental Contamination and Toxicology, Vol. 34, Issue 2
Detection of a key Hg methylation gene, hgcA , in wetland soils : Detection of the Hg methylation gene, hgcA, in soils
journal, January 2014
- Schaefer, J. K.; Kronberg, R. -M.; Morel, F. M. M.
- Environmental Microbiology Reports, Vol. 6, Issue 5
Mercury and Other Heavy Metals Influence Bacterial Community Structure in Contaminated Tennessee Streams
journal, November 2010
- Vishnivetskaya, Tatiana A.; Mosher, Jennifer J.; Palumbo, Anthony V.
- Applied and Environmental Microbiology, Vol. 77, Issue 1
Site-Directed Mutagenesis of HgcA and HgcB Reveals Amino Acid Residues Important for Mercury Methylation
journal, February 2015
- Smith, Steven D.; Bridou, Romain; Johs, Alexander
- Applied and Environmental Microbiology, Vol. 81, Issue 9
MetaVelvet: an extension of Velvet assembler to de novo metagenome assembly from short sequence reads
journal, July 2012
- Namiki, Toshiaki; Hachiya, Tsuyoshi; Tanaka, Hideaki
- Nucleic Acids Research, Vol. 40, Issue 20
Relationships between bacterial energetic metabolism, mercury methylation potential, and hgcA/hgcB gene expression in Desulfovibrio dechloroacetivorans BerOc1
journal, March 2015
- Goñi-Urriza, Marisol; Corsellis, Yannick; Lanceleur, Laurent
- Environmental Science and Pollution Research, Vol. 22, Issue 18
An integrated metagenomics pipeline for strain profiling reveals novel patterns of bacterial transmission and biogeography
journal, October 2016
- Nayfach, Stephen; Rodriguez-Mueller, Beltran; Garud, Nandita
- Genome Research, Vol. 26, Issue 11
The Genetic Basis for Bacterial Mercury Methylation
journal, February 2013
- Parks, J. M.; Johs, A.; Podar, M.
- Science, Vol. 339, Issue 6125, p. 1332-1335
Role of Morphological Growth State and Gene Expression in Desulfovibrio africanus Strain Walvis Bay Mercury Methylation
journal, April 2012
- Moberly, James G.; Miller, Carrie L.; Brown, Steven D.
- Environmental Science & Technology, Vol. 46, Issue 9
Global prevalence and distribution of genes and microorganisms involved in mercury methylation
journal, October 2015
- Podar, Mircea; Gilmour, Cynthia C.; Brandt, Craig C.
- Science Advances, Vol. 1, Issue 9
A new mathematical model for relative quantification in real-time RT-PCR
journal, May 2001
- Pfaffl, M. W.
- Nucleic Acids Research, Vol. 29, Issue 9
Kinetics of Methylmercury Production Revisited
journal, January 2018
- Olsen, Todd A.; Muller, Katherine A.; Painter, Scott L.
- Environmental Science & Technology, Vol. 52, Issue 4
Is total mercury concentration a good predictor of methyl mercury concentration in aquatic systems?
journal, February 1995
- Kelly, Carol A.; Rudd, John W. M.; St. Louis, Vincent L.
- Water, Air, & Soil Pollution, Vol. 80, Issue 1-4
Activated carbon thin-layer placement as an in situ mercury remediation tool in a Penobscot River salt marsh
journal, April 2018
- Gilmour, Cynthia; Bell, Tyler; Soren, Ally
- Science of The Total Environment, Vol. 621
Mercury cycling in surface water, pore water and sediments of Mugu Lagoon, CA, USA
journal, July 2008
- Rothenberg, Sarah E.; Ambrose, Richard F.; Jay, Jennifer A.
- Environmental Pollution, Vol. 154, Issue 1
Improved Yield of High Molecular Weight DNA Coincides with Increased Microbial Diversity Access from Iron Oxide Cemented Sub-Surface Clay Environments
journal, July 2014
- Hurt, Richard A.; Robeson, Michael S.; Shakya, Migun
- PLoS ONE, Vol. 9, Issue 7
Characterizing Microbial Community and Geochemical Dynamics at Hydrothermal Vents Using Osmotically Driven Continuous Fluid Samplers
journal, April 2013
- Robidart, Julie; Callister, Stephen J.; Song, Pengfei
- Environmental Science & Technology, Vol. 47, Issue 9
IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth
journal, April 2012
- Peng, Y.; Leung, H. C. M.; Yiu, S. M.
- Bioinformatics, Vol. 28, Issue 11
Characterization of the Deltaproteobacteria in contaminated and uncontaminated stream sediments and identification of potential mercury methylators
journal, July 2012
- Mosher, Jj; Vishnivetskaya, Ta; Elias, Da
- Aquatic Microbial Ecology, Vol. 66, Issue 3
Persistent Hg contamination and occurrence of Hg-methylating transcript (hgcA) downstream of a chlor-alkali plant in the Olt River (Romania)
journal, December 2015
- Bravo, Andrea G.; Loizeau, Jean-Luc; Dranguet, Perrine
- Environmental Science and Pollution Research, Vol. 23, Issue 11
Sediment-Porewater Partitioning, Total Sulfur, and Methylmercury Production in Estuaries
journal, January 2014
- Schartup, Amina T.; Balcom, Prentiss H.; Mason, Robert P.
- Environmental Science & Technology, Vol. 48, Issue 2
The Pfam protein families database: towards a more sustainable future
journal, December 2015
- Finn, Robert D.; Coggill, Penelope; Eberhardt, Ruth Y.
- Nucleic Acids Research, Vol. 44, Issue D1
The MerR family of transcriptional regulators
journal, June 2003
- Brown, Nigel L.; Stoyanov, Jivko V.; Kidd, Stephen P.
- FEMS Microbiology Reviews, Vol. 27, Issue 2-3
The mcrA gene as an alternative to 16S rRNA in the phylogenetic analysis of methanogen populations in landfill b
journal, November 2002
- Luton, Philip E.; Wayne, Jonathan M.; Sharp, Richard J.
- Microbiology, Vol. 148, Issue 11
Mercury distribution in a polluted marine area, ratio of total mercury, methyl mercury and selenium in sediments, mussels and fish
journal, January 1985
- Mikac, N.; Picer, M.; Stegnar, P.
- Water Research, Vol. 19, Issue 11
Mercury Methylation by the Methanogen Methanospirillum hungatei
journal, August 2013
- Yu, Ri-Qing; Reinfelder, John R.; Hines, Mark E.
- Applied and Environmental Microbiology, Vol. 79, Issue 20
Influence of Dissolved Organic Carbon, pH, and Microbial Respiration Rates on Mercury Methylation and Demethylation in Lake Water
journal, January 1992
- Miskimmin, Brenda M.; Rudd, John W. M.; Kelly, Carol A.
- Canadian Journal of Fisheries and Aquatic Sciences, Vol. 49, Issue 1
Anaerobic Mercury Methylation and Demethylation by Geobacter bemidjiensis Bem
journal, April 2016
- Lu, Xia; Liu, Yurong; Johs, Alexander
- Environmental Science & Technology, Vol. 50, Issue 8
Influence of porewater sulfide on methylmercury production and partitioning in sulfate-impacted lake sediments
journal, February 2017
- Bailey, Logan T.; Mitchell, Carl P. J.; Engstrom, Daniel R.
- Science of The Total Environment, Vol. 580
Comparative Analysis of Pyrosequencing and a Phylogenetic Microarray for Exploring Microbial Community Structures in the Human Distal Intestine
journal, August 2009
- Claesson, Marcus J.; O'Sullivan, Orla; Wang, Qiong
- PLoS ONE, Vol. 4, Issue 8
Dissolved Organic Matter Enhances Microbial Mercury Methylation Under Sulfidic Conditions
journal, February 2012
- Graham, Andrew M.; Aiken, George R.; Gilmour, Cynthia C.
- Environmental Science & Technology, Vol. 46, Issue 5
Analysis of the Microbial Community Structure by Monitoring an Hg Methylation Gene (hgcA) in Paddy Soils along an Hg Gradient
journal, February 2014
- Liu, Y. -R.; Yu, R. -Q.; Zheng, Y. -M.
- Applied and Environmental Microbiology, Vol. 80, Issue 9
Mechanisms of Methyl Mercury Net Degradation in Alder Swamps: The Role of Methanogens and Abiotic Processes
journal, March 2018
- Kronberg, Rose-Marie; Schaefer, Jeffra K.; Björn, Erik
- Environmental Science & Technology Letters, Vol. 5, Issue 4
Role of the Bacterial Organomercury Lyase (MerB) in Controlling Methylmercury Accumulation in Mercury-Contaminated Natural Waters
journal, August 2004
- Schaefer, Jeffra K.; Yagi, Jane; Reinfelder, John R.
- Environmental Science & Technology, Vol. 38, Issue 16
Insights from 20 years of bacterial genome sequencing
journal, February 2015
- Land, Miriam; Hauser, Loren; Jun, Se-Ran
- Functional & Integrative Genomics, Vol. 15, Issue 2
The effects of a stannous chloride-based water treatment system in a mercury contaminated stream
journal, November 2015
- Mathews, Teresa J.; Looney, Brian B.; Bryan, A. Lawrence
- Chemosphere, Vol. 138
Carbon Amendments Alter Microbial Community Structure and Net Mercury Methylation Potential in Sediments
journal, November 2017
- Christensen, Geoff A.; Somenahally, Anil C.; Moberly, James G.
- Applied and Environmental Microbiology, Vol. 84, Issue 3
Methylmercury production in a Chesapeake Bay salt marsh: METHYLMERCURY PRODUCTION IN A SALT MARSH
journal, June 2008
- Mitchell, Carl P. J.; Gilmour, Cynthia C.
- Journal of Geophysical Research: Biogeosciences, Vol. 113, Issue G2
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets
journal, March 2016
- Kumar, Sudhir; Stecher, Glen; Tamura, Koichiro
- Molecular Biology and Evolution, Vol. 33, Issue 7
Microbial mercury methylation in Antarctic sea ice
journal, August 2016
- Gionfriddo, Caitlin M.; Tate, Michael T.; Wick, Ryan R.
- Nature Microbiology, Vol. 1, Issue 10
Bacterial mercury resistance from atoms to ecosystems
journal, June 2003
- Barkay, Tamar; Miller, Susan M.; Summers, Anne O.
- FEMS Microbiology Reviews, Vol. 27, Issue 2-3
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
journal, June 2002
- Vandesompele, Jo; De Preter, Katleen; Pattyn, Filip
- Genome Biology, Vol. 3, Issue 7
mer -Mediated Resistance and Volatilization of Hg(II) Under Anaerobic Conditions
journal, January 2002
- Schaefer, Jeffra K.; Letowski, Jaroslaw; Barkay, Tamar
- Geomicrobiology Journal, Vol. 19, Issue 1
Use of in-field bioreactors demonstrate groundwater filtration influences planktonic bacterial community assembly, but not biofilm composition
journal, March 2018
- Christensen, Geoff A.; Moon, JiWon; Veach, Allison M.
- PLOS ONE, Vol. 13, Issue 3
PEAR: a fast and accurate Illumina Paired-End reAd mergeR
journal, October 2013
- Zhang, J.; Kobert, K.; Flouri, T.
- Bioinformatics, Vol. 30, Issue 5
Mercury Methylation Rates for Geochemically Relevant Hg II Species in Sediments
journal, October 2012
- Jonsson, Sofi; Skyllberg, Ulf; Nilsson, Mats B.
- Environmental Science & Technology, Vol. 46, Issue 21
Archaeal and bacterial communities in three alkaline hot springs in Heart Lake Geyser Basin, Yellowstone National Park
journal, January 2013
- Bowen De León, Kara; Gerlach, Robin; Peyton, Brent M.
- Frontiers in Microbiology, Vol. 4
The Mercury Resistance Operon: From an Origin in a Geothermal Environment to an Efficient Detoxification Machine
journal, January 2012
- Boyd, Eric S.; Barkay, Tamar
- Frontiers in Microbiology, Vol. 3
Active transport, substrate specificity, and methylation of Hg(II) in anaerobic bacteria
journal, May 2011
- Schaefer, J. K.; Rocks, S. S.; Zheng, W.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 21
The metagenomics RAST server – a public resource for the automatic phylogenetic and functional analysis of metagenomes
journal, September 2008
- Meyer, F.; Paarmann, D.; D'Souza, M.
- BMC Bioinformatics, Vol. 9, Issue 1
Spectral Probabilities and Generating Functions of Tandem Mass Spectra: A Strike against Decoy Databases
journal, August 2008
- Kim, Sangtae; Gupta, Nitin; Pevzner, Pavel A.
- Journal of Proteome Research, Vol. 7, Issue 8
FastTree: Computing Large Minimum Evolution Trees with Profiles instead of a Distance Matrix
journal, April 2009
- Price, M. N.; Dehal, P. S.; Arkin, A. P.
- Molecular Biology and Evolution, Vol. 26, Issue 7
MUSCLE: multiple sequence alignment with improved accuracy and speed
conference, January 2004
- Edgar, R. C.
- Proceedings. 2004 IEEE Computational Systems Bioinformatics Conference, 2004. CSB 2004.
Total mercury, methylmercury and selenium in mercury polluted areas in the province Guizhou, China
journal, March 2003
- Horvat, Milena; Nolde, Nataša; Fajon, Vesna
- Science of The Total Environment, Vol. 304, Issue 1-3
Mechanisms Regulating Mercury Bioavailability for Methylating Microorganisms in the Aquatic Environment: A Critical Review
journal, February 2013
- Hsu-Kim, Heileen; Kucharzyk, Katarzyna H.; Zhang, Tong
- Environmental Science & Technology, Vol. 47, Issue 6
Aerobic and Anaerobic Bacterial Mercury Uptake is Driven by Algal Organic Matter Composition and Molecular Weight
journal, November 2018
- Mangal, Vaughn; Stenzler, Benjamin R.; Poulain, Alexandre J.
- Environmental Science & Technology, Vol. 53, Issue 1
Fast, accurate, and lightweight analysis of BS-treated reads with ERNE 2
journal, February 2016
- Prezza, Nicola; Vezzi, Francesco; Käller, Max
- BMC Bioinformatics, Vol. 17, Issue S4
Geobacteraceae are important members of mercury-methylating microbial communities of sediments impacted by waste water releases
journal, January 2018
- Bravo, Andrea G.; Zopfi, Jakob; Buck, Moritz
- The ISME Journal, Vol. 12, Issue 3
Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons
journal, January 2011
- Haas, B. J.; Gevers, D.; Earl, A. M.
- Genome Research, Vol. 21, Issue 3
Sulfate-Reducing Bacteria: Principal Methylators of Mercury in Anoxic Estuarine Sediment †
journal, January 1985
- Compeau, G. C.; Bartha, R.
- Applied and Environmental Microbiology, Vol. 50, Issue 2
Quality-Score Refinement of SSU rRNA Gene Pyrosequencing Differs Across Gene Region for Environmental Samples
journal, April 2012
- Bowen De León, Kara; Ramsay, Bradley D.; Fields, Matthew W.
- Microbial Ecology, Vol. 64, Issue 2
Methylmercury Decomposition in Sediments and Bacterial Cultures: Involvement of Methanogens and Sulfate Reducers in Oxidative Demethylation
journal, January 1991
- Oremland, Ronald S.; Culbertson, Charles W.; Winfrey, Michael R.
- Applied and Environmental Microbiology, Vol. 57, Issue 1
Works referencing / citing this record:
Biotic formation of methylmercury: A bio–physico–chemical conundrum
journal, November 2019
- Bravo, Andrea G.; Cosio, Claudia
- Limnology and Oceanography, Vol. 65, Issue 5
An Improved hgcAB Primer Set and Direct High-Throughput Sequencing Expand Hg-Methylator Diversity in Nature
journal, October 2020
- Gionfriddo, Caitlin M.; Wymore, Ann M.; Jones, Daniel S.
- Frontiers in Microbiology, Vol. 11
Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.