Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery
Abstract
Serial femtosecond crystallography (SFX) using X-ray Free-Electron Lasers (XFELs) allows for room temperature protein structure determination without evidence of conventional radiation damage. In this method, a liquid suspension of protein microcrystals can be delivered to the X-ray beam in vacuum as a micro-jet, which replenishes the crystals at a rate that exceeds the current XFEL pulse repetition rate. Gas dynamic virtual nozzles produce the required micrometer-sized streams by the focusing action of a coaxial sheath gas and have been shown to be effective for SFX experiments. Here, we describe the design and characterization of such nozzles assembled from ceramic micro-injection molded outer gas-focusing capillaries. Trends of the emitted jet diameter and jet length as a function of supplied liquid and gas flow rates are measured by a fast imaging system. The observed trends are explained by derived relationships considering choked gas flow and liquidflow conservation. In conclusion, the performance of these nozzles in a SFX experiment is presented, including an analysis of the observed background.
- Authors:
-
[1];
[3];
[1]
- Deutsches Elektronen-Synchrotron, Hamburg (Germany)
- Deutsches Elektronen-Synchrotron, Hamburg (Germany); Arizona State Univ., Tempe, AZ (United States)
- Univ. of Hamburg, Hamburg (Germany)
- Helmholtz-Zentrum Geesthacht, Max-Planck-StraBe, Geesthacht (Germany)
- Deutsches Elektronen-Synchrotron, Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany); Centre for Ultrafast Imaging, Hamburg (Germany)
- Publication Date:
- Research Org.:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1234709
- Grant/Contract Number:
- AC02-76SF00515
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Review of Scientific Instruments
- Additional Journal Information:
- Journal Volume: 86; Journal Issue: 12; Journal ID: ISSN 0034-6748
- Publisher:
- American Institute of Physics (AIP)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; ceramics; gas liquid flows; x-ray scattering; proteins; fluid drops
Citation Formats
Beyerlein, K. R., Adriano, L., Heymann, M., Kirian, R., Knoska, J., Wilde, F., Chapman, H. N., and Bajt, S. Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery. United States: N. p., 2015.
Web. doi:10.1063/1.4936843.
Beyerlein, K. R., Adriano, L., Heymann, M., Kirian, R., Knoska, J., Wilde, F., Chapman, H. N., & Bajt, S. Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery. United States. https://doi.org/10.1063/1.4936843
Beyerlein, K. R., Adriano, L., Heymann, M., Kirian, R., Knoska, J., Wilde, F., Chapman, H. N., and Bajt, S. Tue .
"Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery". United States. https://doi.org/10.1063/1.4936843. https://www.osti.gov/servlets/purl/1234709.
@article{osti_1234709,
title = {Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery},
author = {Beyerlein, K. R. and Adriano, L. and Heymann, M. and Kirian, R. and Knoska, J. and Wilde, F. and Chapman, H. N. and Bajt, S.},
abstractNote = {Serial femtosecond crystallography (SFX) using X-ray Free-Electron Lasers (XFELs) allows for room temperature protein structure determination without evidence of conventional radiation damage. In this method, a liquid suspension of protein microcrystals can be delivered to the X-ray beam in vacuum as a micro-jet, which replenishes the crystals at a rate that exceeds the current XFEL pulse repetition rate. Gas dynamic virtual nozzles produce the required micrometer-sized streams by the focusing action of a coaxial sheath gas and have been shown to be effective for SFX experiments. Here, we describe the design and characterization of such nozzles assembled from ceramic micro-injection molded outer gas-focusing capillaries. Trends of the emitted jet diameter and jet length as a function of supplied liquid and gas flow rates are measured by a fast imaging system. The observed trends are explained by derived relationships considering choked gas flow and liquidflow conservation. In conclusion, the performance of these nozzles in a SFX experiment is presented, including an analysis of the observed background.},
doi = {10.1063/1.4936843},
journal = {Review of Scientific Instruments},
number = 12,
volume = 86,
place = {United States},
year = {Tue Dec 08 00:00:00 EST 2015},
month = {Tue Dec 08 00:00:00 EST 2015}
}
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Works referenced in this record:
Femtosecond X-ray protein nanocrystallography
journal, February 2011
- Chapman, Henry N.; Fromme, Petra; Barty, Anton
- Nature, Vol. 470, Issue 7332, p. 73-77
Potential for biomolecular imaging with femtosecond X-ray pulses
journal, August 2000
- Neutze, Richard; Wouts, Remco; van der Spoel, David
- Nature, Vol. 406, Issue 6797
Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements
journal, December 2011
- Barty, Anton; Caleman, Carl; Aquila, Andrew
- Nature Photonics, Vol. 6, Issue 1
Serial Femtosecond Crystallography of G Protein-Coupled Receptors
journal, December 2013
- Liu, W.; Wacker, D.; Gati, C.
- Science, Vol. 342, Issue 6165
High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography
journal, May 2012
- Boutet, S.; Lomb, L.; Williams, G. J.
- Science, Vol. 337, Issue 6092
Natively Inhibited Trypanosoma brucei Cathepsin B Structure Determined by Using an X-ray Laser
journal, November 2012
- Redecke, L.; Nass, K.; DePonte, D. P.
- Science, Vol. 339, Issue 6116
Native structure of photosystem II at 1.95 Å resolution viewed by femtosecond X-ray pulses
journal, November 2014
- Suga, Michihiro; Akita, Fusamichi; Hirata, Kunio
- Nature, Vol. 517, Issue 7532
De novo protein crystal structure determination from X-ray free-electron laser data
journal, November 2013
- Barends, Thomas R. M.; Foucar, Lutz; Botha, Sabine
- Nature, Vol. 505, Issue 7482
Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography
journal, December 2013
- Johansson, Linda C.; Arnlund, David; Katona, Gergely
- Nature Communications, Vol. 4, Issue 1
Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
journal, August 2014
- Arnlund, David; Johansson, Linda C.; Wickstrand, Cecilia
- Nature Methods, Vol. 11, Issue 9
Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser
journal, July 2014
- Kupitz, Christopher; Basu, Shibom; Grotjohann, Ingo
- Nature, Vol. 513, Issue 7517
Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature
journal, February 2013
- Kern, J.; Alonso-Mori, R.; Tran, R.
- Science, Vol. 340, Issue 6131
Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein
journal, December 2014
- Tenboer, Jason; Basu, Shibom; Zatsepin, Nadia
- Science, Vol. 346, Issue 6214
Liquid sample delivery techniques for serial femtosecond crystallography
journal, July 2014
- Weierstall, Uwe
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647
Gas dynamic virtual nozzle for generation of microscopic droplet streams
journal, September 2008
- DePonte, D. P.; Weierstall, U.; Schmidt, K.
- Journal of Physics D: Applied Physics, Vol. 41, Issue 19, Article No. 195505
Injector for scattering measurements on fully solvated biospecies
journal, March 2012
- Weierstall, U.; Spence, J. C. H.; Doak, R. B.
- Review of Scientific Instruments, Vol. 83, Issue 3
Focusing liquid microjets with nozzles
journal, May 2012
- Acero, A. J.; Ferrera, C.; Montanero, J. M.
- Journal of Micromechanics and Microengineering, Vol. 22, Issue 6
Revision of capillary cone-jet physics: Electrospray and flow focusing
journal, June 2009
- Gañán-Calvo, Alfonso M.; Montanero, José M.
- Physical Review E, Vol. 79, Issue 6
Micrometer glass nozzles for flow focusing
journal, June 2010
- Montanero, J. M.; Gañán-Calvo, A. M.; Acero, A. J.
- Journal of Micromechanics and Microengineering, Vol. 20, Issue 7
Global stability of the focusing effect of fluid jet flows
journal, March 2011
- Montanero, J. M.; Rebollo-Muñoz, N.; Herrada, M. A.
- Physical Review E, Vol. 83, Issue 3
Global and local instability of flow focusing: The influence of the geometry
journal, June 2010
- Vega, Emilio J.; Montanero, José M.; Herrada, Miguel A.
- Physics of Fluids, Vol. 22, Issue 6
Microfluidic liquid jet system with compatibility for atmospheric and high-vacuum conditions
journal, January 2014
- Trebbin, Martin; Krüger, Kilian; DePonte, Daniel
- Lab Chip, Vol. 14, Issue 10
On the validity of a universal solution for viscous capillary jets
journal, December 2011
- Montanero, J. M.; Herrada, M. A.; Ferrera, C.
- Physics of Fluids, Vol. 23, Issue 12
Modes in flow focusing and instability of coaxial liquid–gas jets
journal, June 2009
- Si, Ting; Li, Fang; Yin, Xie-Yuan
- Journal of Fluid Mechanics, Vol. 629
The Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS)
journal, March 2010
- Boutet, Sébastien; J. Williams, Garth
- New Journal of Physics, Vol. 12, Issue 3
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
journal, February 2014
- Weierstall, Uwe; James, Daniel; Wang, Chong
- Nature Communications, Vol. 5, Issue 1
Works referencing / citing this record:
Simulation of liquid micro-jet in free expanding high-speed co-flowing gas streams
journal, August 2018
- Zahoor, Rizwan; Belšak, Grega; Bajt, Saša
- Microfluidics and Nanofluidics, Vol. 22, Issue 8
Ultracompact 3D microfluidics for time-resolved structural biology
journal, January 2020
- Knoška, Juraj; Adriano, Luigi; Awel, Salah
- Nature Communications, Vol. 11, Issue 1
Mixing injector enables time-resolved crystallography with high hit rate at X-ray free electron lasers
journal, August 2016
- Calvey, George D.; Katz, Andrea M.; Schaffer, Chris B.
- Structural Dynamics, Vol. 3, Issue 5
3D printed nozzles on a silicon fluidic chip
journal, March 2019
- Bohne, Sven; Heymann, Michael; Chapman, Henry N.
- Review of Scientific Instruments, Vol. 90, Issue 3
Controlled beams of shock-frozen, isolated, biological and artificial nanoparticles
journal, March 2020
- Samanta, Amit K.; Amin, Muhamed; Estillore, Armando D.
- Structural Dynamics, Vol. 7, Issue 2
3D printed droplet generation devices for serial femtosecond crystallography enabled by surface coating
journal, August 2019
- Echelmeier, Austin; Kim, Daihyun; Cruz Villarreal, Jorvani
- Journal of Applied Crystallography, Vol. 52, Issue 5
De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography
journal, August 2018
- Botha, S.; Baitan, D.; Jungnickel, K. E. J.
- IUCrJ, Vol. 5, Issue 5
Sample delivery for serial crystallography at free-electron lasers and synchrotrons
journal, January 2019
- Grünbein, Marie Luise; Nass Kovacs, Gabriela
- Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2
A guide to sample delivery systems for serial crystallography
journal, August 2019
- Zhao, Feng‐Zhu; Zhang, Bin; Yan, Er‐Kai
- The FEBS Journal, Vol. 286, Issue 22
Three-dimensional-printed gas dynamic virtual nozzles for x-ray laser sample delivery
journal, January 2016
- Nelson, Garrett; Kirian, Richard A.; Weierstall, Uwe
- Optics Express, Vol. 24, Issue 11
Light-sheet imaging for the recording of transverse absolute density distributions of gas-phase particle-beams from nanoparticle injectors
journal, January 2019
- Worbs, Lena; Lübke, Jannik; Roth, Nils
- Optics Express, Vol. 27, Issue 25
Jet delivery system for Raman scattering on bio-inorganic compounds
text, January 2016
- Wetzel, Alina; Biebl, F.; Beyerlein, K. R.
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Rapid Sample Delivery for Megahertz Serial Crystallography at X-ray FELs
text, January 2018
- Wiedorn, Max O.; Awel, Salah; Morgan, Andrew J.
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Megahertz serial crystallography
text, January 2018
- Wiedorn, Max O.; Oberthür, Dominik; Bean, Richard
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Double-flow focused liquid injector for efficient serial femtosecond crystallography
journal, March 2017
- Oberthuer, Dominik; Knoška, Juraj; Wiedorn, Max O.
- Scientific Reports, Vol. 7, Issue 1
Jet delivery system for Raman scattering on bio-inorganic compounds
journal, November 2016
- Wetzel, A.; Biebl, F.; Beyerlein, K. R.
- Applied Physics Letters, Vol. 109, Issue 21
Rapid sample delivery for megahertz serial crystallography at X-ray FELs
journal, July 2018
- Wiedorn, Max O.; Awel, Salah; Morgan, Andrew J.
- IUCrJ, Vol. 5, Issue 5
3D printed nozzles on a silicon fluidic chip
text, January 2019
- Bohne, Sven; Heymann, Michael; Chapman, Henry N.
- American Institute of Physics
Ultracompact 3D microfluidics for time-resolved structural biology
text, January 2020
- Knoška, Juraj; Adriano, Luigi; Awel, Salah
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Megahertz serial crystallography
journal, October 2018
- Wiedorn, Max O.; Oberthür, Dominik; Bean, Richard
- Nature Communications, Vol. 9, Issue 1
Rapid sample delivery for megahertz serial crystallography at X-ray FELs
text, January 2018
- Wiedorn, Max Oliver; Awel, Salah; Morgan, Andrew J.
- TUHH Universitätsbibliothek
Ultracompact 3D microfluidics for time-resolved structural biology
text, January 2020
- Knoska, Juraj; Adriano, Luigi; Awel, Salah
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Megahertz serial crystallography
text, January 2018
- Wiedorn, Max Oliver; Oberthür, Dominik; Bean, Richard
- Nature Publishing Group UK
De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography
text, January 2018
- Botha, S.; Baitan, D.; Jungnickel, K. E. J.
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Light-sheet imaging for the recording of transverse absolute density distributions of gas-phase particle-beams from nanoparticle injectors
text, January 2019
- Worbs, Lena; Lübke, Jannik; Roth, Nils
- arXiv
Ultracompact 3D microfluidics for time-resolved structural biology
journal, January 2020
- Knoška, Juraj; Adriano, Luigi; Awel, Salah
- Nature Communications, Vol. 11, Issue 1
Mixing injector enables time-resolved crystallography with high hit rate at X-ray free electron lasers
journal, August 2016
- Calvey, George D.; Katz, Andrea M.; Schaffer, Chris B.
- Structural Dynamics, Vol. 3, Issue 5
Controlled beams of shock-frozen, isolated, biological and artificial nanoparticles
text, January 2020
- Amin, Muhamed; Estillore, Armando; Roth, Nils
- Deutsches Elektronen-Synchrotron, DESY, Hamburg
Laser-induced acoustic desorption of thermally stable and unstable biomolecules
preprint, January 2018
- Huang, Zhipeng; Horke, Daniel A.; Küpper, Jochen
- arXiv
Dripping, jetting and tip streaming
preprint, January 2019
- Montanero, Jose M.; Ganan-Calvo, Alfonso M.
- arXiv
Light-sheet imaging for the recording of transverse absolute density distributions of gas-phase particle-beams from nanoparticle injectors
text, January 2019
- Worbs, Lena; Lübke, Jannik; Roth, Nils
- arXiv
Controlled beams of shockfrozen, isolated, biological and artificial nanoparticles
preprint, January 2019
- Samanta, Amit K.; Amin, Muhamed; Estillore, Armando D.
- arXiv