The effect of retinal pigment epithelial cell patch size on growth factor expression
Abstract
The spatial organization of retinal pigment epithelial (RPE) cells grown in culture was controlled using micropatterning techniques in order to examine the effect of patch size on cell health and differentiation. Understanding this effect is a critical step in the development of multiplexed high throughput fluidic assays and provides a model for replicating disease states associated with the deterioration of retinal tissue during age-related macular degeneration (AMD). Microcontact printing of fibronectin on polystyrene and glass substrates was used to promote cell attachment, forming RPE patches of controlled size and shape. These colonies mimic the effect of atrophy and loss-of-function that occurs in the retina during degenerative diseases such as AMD. After 72 hours of cell growth, levels of vascular endothelial growth factor (VEGF), an important biomarker of AMD, were measured. Cells were counted and morphological indicators of cell viability and tight junction formation were assessed via fluorescence microscopy. As a result, up to a twofold increase of VEGF expression per cell was measured as colony size decreased, suggesting that the local microenvironment of, and connections between, RPE cells influences growth factor expression leading to the initiation and progression of diseases such as AMD.
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1185385
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biomaterials
- Additional Journal Information:
- Journal Volume: 35; Journal Issue: 13; Journal ID: ISSN 0142-9612
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; microcontact printing; retinal pigment epithelial; tight junctions; VEGF; in vitro model; micropatterning
Citation Formats
Vargis, Elizabeth A., Peterson, Cristen B., Morrell-Falvey, Jennifer L., Retterer, Scott T., and Collier, Charles Patrick. The effect of retinal pigment epithelial cell patch size on growth factor expression. United States: N. p., 2014.
Web. doi:10.1016/j.biomaterials.2014.01.016.
Vargis, Elizabeth A., Peterson, Cristen B., Morrell-Falvey, Jennifer L., Retterer, Scott T., & Collier, Charles Patrick. The effect of retinal pigment epithelial cell patch size on growth factor expression. United States. https://doi.org/10.1016/j.biomaterials.2014.01.016
Vargis, Elizabeth A., Peterson, Cristen B., Morrell-Falvey, Jennifer L., Retterer, Scott T., and Collier, Charles Patrick. Thu .
"The effect of retinal pigment epithelial cell patch size on growth factor expression". United States. https://doi.org/10.1016/j.biomaterials.2014.01.016. https://www.osti.gov/servlets/purl/1185385.
@article{osti_1185385,
title = {The effect of retinal pigment epithelial cell patch size on growth factor expression},
author = {Vargis, Elizabeth A. and Peterson, Cristen B. and Morrell-Falvey, Jennifer L. and Retterer, Scott T. and Collier, Charles Patrick},
abstractNote = {The spatial organization of retinal pigment epithelial (RPE) cells grown in culture was controlled using micropatterning techniques in order to examine the effect of patch size on cell health and differentiation. Understanding this effect is a critical step in the development of multiplexed high throughput fluidic assays and provides a model for replicating disease states associated with the deterioration of retinal tissue during age-related macular degeneration (AMD). Microcontact printing of fibronectin on polystyrene and glass substrates was used to promote cell attachment, forming RPE patches of controlled size and shape. These colonies mimic the effect of atrophy and loss-of-function that occurs in the retina during degenerative diseases such as AMD. After 72 hours of cell growth, levels of vascular endothelial growth factor (VEGF), an important biomarker of AMD, were measured. Cells were counted and morphological indicators of cell viability and tight junction formation were assessed via fluorescence microscopy. As a result, up to a twofold increase of VEGF expression per cell was measured as colony size decreased, suggesting that the local microenvironment of, and connections between, RPE cells influences growth factor expression leading to the initiation and progression of diseases such as AMD.},
doi = {10.1016/j.biomaterials.2014.01.016},
journal = {Biomaterials},
number = 13,
volume = 35,
place = {United States},
year = {Thu Jan 30 00:00:00 EST 2014},
month = {Thu Jan 30 00:00:00 EST 2014}
}
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