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Title: Structural hysteresis in dragline spider silks induced by supercontraction: an X-ray fiber micro-diffraction study

Abstract

Interaction with water causes shrinkage and significant changes in the structure of spider dragline silks, which has been referred to as supercontraction in the literature. Preferred orientation or alignment of protein chains with respect to the fiber axis is extensively changed during this supercontraction process. Synchrotron X-ray micro-fiber diffraction experiments have been performed on Nephila clavipes and Argiope aurantia major and minor ampullate dragline spider fibers in the native dry, contracted (by immersion in water) and restretched (from contracted) states. Changes in the orientation of β-sheet nanocrystallites and the oriented component of the amorphous network have been determined from wide-angle X-ray diffraction patterns. While both the crystalline and amorphous components lose preferred orientation on wetting with water, the nano-crystallites regain their orientation on wet-restretching, whereas the oriented amorphous components only partially regain their orientation. Dragline major ampullate silks in both the species contract more than their minor ampullate silks.

Authors:
 [1];  [2]
+ Show Author Affiliations
  1. Univ. of Wisconsin-Milwaukee, WI (United States). Dept. of Physics; Arizona State Univ., Tempe, AZ (United States). Dept. of Chemistry and Biochemistry
  2. Arizona State Univ., Tempe, AZ (United States). Dept. of Chemistry and Biochemistry
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOD; National Science Foundation (NSF)
OSTI Identifier:
1168489
Grant/Contract Number:  
AC02-06CH11357; SC0004791
Resource Type:
Accepted Manuscript
Journal Name:
RSC Adv.
Additional Journal Information:
Journal Volume: 5; Journal Issue: (2) ; 2015
Country of Publication:
United States
Language:
ENGLISH
Subject:
36 MATERIALS SCIENCE

Citation Formats

Sampath, Sujatha, and Yarger, Jeffery L. Structural hysteresis in dragline spider silks induced by supercontraction: an X-ray fiber micro-diffraction study. United States: N. p., 2014. Web. doi:10.1039/C4RA13936D.
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Sampath, Sujatha, & Yarger, Jeffery L. Structural hysteresis in dragline spider silks induced by supercontraction: an X-ray fiber micro-diffraction study. United States. https://doi.org/10.1039/C4RA13936D
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Sampath, Sujatha, and Yarger, Jeffery L. Thu . "Structural hysteresis in dragline spider silks induced by supercontraction: an X-ray fiber micro-diffraction study". United States. https://doi.org/10.1039/C4RA13936D. https://www.osti.gov/servlets/purl/1168489.
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@article{osti_1168489,
title = {Structural hysteresis in dragline spider silks induced by supercontraction: an X-ray fiber micro-diffraction study},
author = {Sampath, Sujatha and Yarger, Jeffery L.},
abstractNote = {Interaction with water causes shrinkage and significant changes in the structure of spider dragline silks, which has been referred to as supercontraction in the literature. Preferred orientation or alignment of protein chains with respect to the fiber axis is extensively changed during this supercontraction process. Synchrotron X-ray micro-fiber diffraction experiments have been performed on Nephila clavipes and Argiope aurantia major and minor ampullate dragline spider fibers in the native dry, contracted (by immersion in water) and restretched (from contracted) states. Changes in the orientation of β-sheet nanocrystallites and the oriented component of the amorphous network have been determined from wide-angle X-ray diffraction patterns. While both the crystalline and amorphous components lose preferred orientation on wetting with water, the nano-crystallites regain their orientation on wet-restretching, whereas the oriented amorphous components only partially regain their orientation. Dragline major ampullate silks in both the species contract more than their minor ampullate silks.},
doi = {10.1039/C4RA13936D},
journal = {RSC Adv.},
number = (2) ; 2015,
volume = 5,
place = {United States},
year = {Thu Nov 27 00:00:00 EST 2014},
month = {Thu Nov 27 00:00:00 EST 2014}
}
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https://doi.org/10.1039/C4RA13936D
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