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Title: Biological imaging by soft x-ray diffraction microscopy

We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [4] ;  [3] ;  [5] ;  [1] ;  [1] ;  [6] ;  [1]
  1. Stony Brook Univ., Stony Brook, NY (United States)
  2. Cornell Univ., Ithaca, NY (United States)
  3. Stony Brook Univ., Stony Brook, NY (United States); Brookhaven National Lab., Upton, NY (United States). Center for Functional Nanomaterials
  4. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source
  5. Stony Brook Univ., Stony Brook, NY (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source
  6. State Univ. of New York at Stony Brook, NY (United States)
Publication Date:
OSTI Identifier:
1164139
Grant/Contract Number:
FG02-04ER46128
Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 102; Journal Issue: 43; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Research Org:
Stony Brook Univ., Stony Brook, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY