skip to main content

DOE PAGESDOE PAGES

Title: Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell

We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below -170 °C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [1] ;  [3] ;  [2] ;  [1] ;  [1] ;  [1] ;  [1]
  1. Stony Brook Univ., NY (United States) Dept. of Physics and Astronomy
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Stony Brook Univ., NY (United States) Dept. of Biochemistry and Cell Biology
Publication Date:
OSTI Identifier:
1164142
Grant/Contract Number:
FG02-04ER46128
Type:
Accepted Manuscript
Journal Name:
Physical Review Letters
Additional Journal Information:
Journal Volume: 103; Journal Issue: 19; Journal ID: ISSN 0031-9007
Publisher:
American Physical Society (APS)
Research Org:
Stony Brook Univ., NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY