Title: Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination

Abstract

Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.

Inventors:

Xie, Jianming; Wang, Lei; Wu, Ning; Schultz, Peter G.

Issue Date:

Tue Jul 15 00:00:00 EDT 2008

Research Org.:

The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

984459

Patent Number(s):

7399619

Application Number:

11/137,850

Assignee:

The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

C07H21/04 - with deoxyribosyl as saccharide radical

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/67 - General methods for enhancing the expression
C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P21/02 - having a known sequence of two or more amino acids, e.g. glutathione

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DOE Contract Number:  

FG02-03ER46051

Resource Type:

Patent

Resource Relation:

Patent File Date: 2005 May 24

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES