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Title: Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof

Abstract

Glutamine phenylpyruvate transaminase (GPT) proteins, nucleic acid molecules encoding GPT proteins, and uses thereof are disclosed. Provided herein are various GPT proteins and GPT gene coding sequences isolated from a number of plant species. As disclosed herein, GPT proteins share remarkable structural similarity within plant species, and are active in catalyzing the synthesis of 2-hydroxy-5-oxoproline (2-oxoglutaramate), a powerful signal metabolite which regulates the function of a large number of genes involved in the photosynthesis apparatus, carbon fixation and nitrogen metabolism.

Inventors:
; ;
Issue Date:
Research Org.:
Los Alamos National Security, LLC, Los Alamos, NM (United States); University of Maine System Board of Trustees, Bangor, ME (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1493301
Patent Number(s):
10119127
Application Number:
15/064,329
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM); University of Maine System Board of Trustees (Bangor, ME)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
DOE Contract Number:  
W-7405-ENG-36; AC52-06NA25396
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Mar 08
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Unkefer, Pat J., Anderson, Penelope S., and Knight, Thomas J. Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof. United States: N. p., 2018. Web.
Unkefer, Pat J., Anderson, Penelope S., & Knight, Thomas J. Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof. United States.
Unkefer, Pat J., Anderson, Penelope S., and Knight, Thomas J. Tue . "Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof". United States. https://www.osti.gov/servlets/purl/1493301.
@article{osti_1493301,
title = {Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof},
author = {Unkefer, Pat J. and Anderson, Penelope S. and Knight, Thomas J.},
abstractNote = {Glutamine phenylpyruvate transaminase (GPT) proteins, nucleic acid molecules encoding GPT proteins, and uses thereof are disclosed. Provided herein are various GPT proteins and GPT gene coding sequences isolated from a number of plant species. As disclosed herein, GPT proteins share remarkable structural similarity within plant species, and are active in catalyzing the synthesis of 2-hydroxy-5-oxoproline (2-oxoglutaramate), a powerful signal metabolite which regulates the function of a large number of genes involved in the photosynthesis apparatus, carbon fixation and nitrogen metabolism.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 06 00:00:00 EST 2018},
month = {Tue Nov 06 00:00:00 EST 2018}
}

Works referenced in this record:

Biosynthesis and secretion of mugineic acid family phytosiderophores in zinc-deficient barley
journal, October 2006


Structural Insight into the Mechanism of Substrate Specificity of Aedes Kynurenine Aminotransferase
journal, February 2008


Omega-amidase pathway in the degradation of glutamine in Neurospora crassa.
journal, January 1985


Dual promoter of Agrobacterium tumefaciens mannopine synthase genes is regulated by plant growth hormones
journal, May 1989


Plant Glutamine Phenylpyruvate Transaminase Gene and Transgenic Plants Carrying Same
patent-application, October 2010


Genetic Analysis of Protein Stability and Function
journal, December 1989


Drug metabolizing enzymes
patent-application, June 2004


Transgenic plants that exhibit enhanced nitrogen assimilation
patent-application, June 2002


Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
patent-application, February 2007


Molecules for diagnostics and therapeutics
patent-application, January 2004


Maize α-tubulin genes are expressed according to specific patterns of cell differentiation
journal, January 1998


[21] Electroporation of DNA and RNA into plant protoplasts
book, January 1987


Targeting transgene expression in research, agricultural, and environmental applications: Promoters used in plant transformation
journal, January 2004

  • Potenza, Carol; Aleman, Lorenzo; Sengupta-Gopalan, Champa
  • In Vitro Cellular & Developmental Biology - Plant, Vol. 40, Issue 1, p. 1-22
  • https://doi.org/10.1079/IVP2003477

Use of prolines for improving growth and other properties of plants and algae
patent, December 2004


Use of prolines for improving growth and other properties of plants and algae
patent, April 2003


Method of treating actinic keratosis with 3.75% imiquimod cream
patent, October 2012


Efficient transformation of Arabidopsis thaliana: comparison of the efficiencies with various organs, plant ecotypes and Agrobacterium strains
journal, December 1992


25324, 50287, 28899, 47007, and 42967 transferase family members and uses therefor
patent-application, February 2002


The Molecular-Genetics of Nitrogen Assimilation Into Amino Acids In Higher Plants
journal, June 1996


Nucleic Acids Encoding Plant Glutamine Phenylpyruvate Transaminase (GPT) and Uses Thereof
patent-application, August 2013


Preparation of 2-hydroxy-5-oxoproline and analogs thereof
patent, September 2001


Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris
journal, July 1986


Tissue- and cell-specific activity of a phenylalanine ammonia-lyase promoter in transgenic plants.
journal, July 1989


Enzymes
patent-application, September 2005


Enzyme Redundancy and the Importance of 2-Oxoglutarate in Higher Plant Ammonium Assimilation
journal, July 2000


Genetic transformation of plants by protoplast electroporation
journal, October 1994


Modeling and molecular dynamics of glutamine transaminase K/cysteine conjugate β-lyase
journal, September 2003


Molecular analysis of the alcohol dehydrogenase (Adhl) gene of maize
journal, May 1984


Oats Tolerant of Pseudomonas syringae pv. tabaci Contain Tabtoxinine-β-Lactam-Insensitive Leaf Glutamine Synthetases
journal, October 1988


Transgenic plants that exhibit enhanced nitrogen assimilation
patent, August 2000


Synthetic promoters genetic control through cis engineering
journal, March 2007


Plant glutamine synthetase complements a glnA mutation in Escherichia coli
journal, June 1986


A Simple and General Method for Transferring Genes into Plants
journal, March 1985


An Agrobacterium -transformed cell culture from the monocot Asparagus officinalis
journal, December 1984


A Rapid and Simple Method of Assaying Plants Transformed with Hygromycin or PPT Resistance Genes
journal, January 1997


Expression of soybean lectin gene deletions in tobacco
journal, January 1990


Glutamine synthetase isoenzymes of Phaseolus vulgaris L. subunit composition in developing root nodules and plumules
journal, November 1989


Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof
patent, March 2016


Amino acid substitution matrices from protein blocks.
journal, November 1992


Use of prolines for improving growth and/or yield
patent-application, May 2007


Light-Regulated and Cell-Specific Expression of Tomato rbcS-gusA and Rice rbcS-gusA Fusion Genes in Transgenic Rice
journal, July 1993


Transgenic plants with enhanced growth characteristics
patent, September 2016


Transgenic Algae Engineered for Higher Performance
patent-application, September 2011


Use of prolines for improving growth and other properties of plants and algae
patent, July 2003


Plants having enhanced nitrogen assimilation/metabolism
patent-application, January 2005


Overproduction of alfalfa glutamine synthetase in transgenic tobacco plants
journal, June 1989


Crystal Structure of Human Kynurenine Aminotransferase I
journal, September 2004


Characterization of diphtheria fusion proteins targeted to the human interleukin-3 receptor
journal, August 2000


A gene network controlling glutamine and asparagine biosynthesis in plants.
journal, November 1991


Improved tools for biological sequence comparison.
journal, April 1988


Overexpression of Cytosolic Glutamine Synthetase. Relation to Nitrogen, Light, and Photorespiration
journal, June 2002


A Highly Efficient Transformation Protocol for Micro-Tom, a Model Cultivar for Tomato Functional Genomics
journal, March 2006


Structural and transcriptional analysis of DNA sequences flanking genes that encode 19 kilodalton zeins
journal, April 1987


Fertile transgenic rice plants regenerated from transformed protoplasts
journal, March 1989


Expression of a conifer glutamine synthetase gene in transgenic poplar
journal, November 1999


Modulation of glutamine synthetase gene expression in tobacco by the introduction of an alfalfa glutamine synthetase gene in sense and antisense orientation: molecular and biochemical analysis
journal, January 1993


Direct gene transfer to plants
journal, December 1984


Plastid transformation
patent-application, December 2004


Carbon and Amino Acids Reciprocally Modulate the Expression of Glutamine Synthetase in Arabidopsis
journal, September 1999


A simple method for the production of highly competent cells ofAgrobacterium for transformation via electroporation
journal, April 1998


Does root glutamine synthetase control plant biomass production in Lotus japonicus L.?
journal, October 1999


Expression of genes transferred into monocot and dicot plant cells by electroporation.
journal, September 1985


Transgenic Algae Engineered for Higher Performance
patent-application, May 2016


Transgenic plants with enhanced growth characteristics
patent-application, February 2011


Sequence of a 21 kd zein gene from maize containing an in-frame stop codon
journal, January 1989


Light Regulation of Gene Expression in Higher Plants
journal, June 1985


Vascular expression of a bean cell wall glycine-rich protein-β-glucuronidase gene fusion in transgenic tobacco
journal, May 1989


Cysteine-S-conjugate β-Lyase Activity and Pyridoxal Phosphate Binding Site of Onion Alliin Lyase
journal, January 1997


Method of Treating Actinic Keratosis with 3.75% Imiquimod Cream
patent-application, October 2011


Nucleic Acids and Proteins and Methods for Making and Using Them
patent-application, January 2010


GATEWAY™ vectors for Agrobacterium-mediated plant transformation
journal, May 2002


A zein gene of maize is transcribed from two widely separated promoter regions
journal, October 1983


Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
patent-application, May 2006


Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993


The glutamine synthetase gene family of Arabidopsis thaliana light-regulation and differential expression in leaves, roots and seeds
journal, November 1991


Electrotransformation of Chlorella vulgaris
journal, May 1999


Lower Dosage Strength Imiquimod Formulations and Shorter Dosing Regimens for Treating Actinic Keratoses
patent-application, January 2011


Characterization of an rbcS gene from Nicotiana plumbaginifolia and expression of an rbcS-CAT chimeric gene in homologous and heterologous nuclear background
journal, November 1986


Rice nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
patent-application, June 2004


Increasing plant growth by modulating omega-amidase expression in plants
patent, June 2015


Structure and expression of the maize gene encoding the phosphoenolpyruvate carboxylase isozyme involved in C4 photosynthesis
journal, May 1989


The Glutamine Transaminase-ω-Amidase Pathwa
journal, January 1977


Transgenic Plants with Enhanced Growth Characteristics
patent-application, July 2010


Molecular approaches to the study of amino acid biosynthesis in plants
journal, January 1991


Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer
journal, January 1985


Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase.
journal, May 1990


Transgenic Plants with Enhanced Growth Characteristics
patent-application, September 2013


Lower Dosage Strength Pharmaceutical Compositions Formulated with 2.5% Imiquimod
patent-application, October 2011


Preferential Expression of an α-Tubulin Gene of Arabidopsis in Pollen
journal, May 1992


Photosynthesis-Associated Gene Families: Differences in Response to Tissue-Specific and Environmental Factors
journal, July 1986


Technical Focus: A guide to Agrobacterium binary Ti vectors
journal, October 2000


Developmentally Regulated Expression of the Gene Family for Cytosolic Glutamine Synthetase in Pisum sativum
journal, October 1989


A promoter sequence involved in cell-specific expression of the pea glutamine synthetase GS3A gene in organs of transgenic tobacco and alfalfa
journal, September 1991


Enhanced evolutionary PCR using oligonucleotides with inosine at the 3′-terminus
journal, January 1991


Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
journal, January 1988


Genes and uses for plant improvement
patent-application, April 2006


Molecular cloning and expression of a cDNA for human kidney cysteine conjugate β-lyase
journal, March 1995


Molecular analysis of three maize 22 kDa auxin-binding protein genes - transient promoter expression and regulatory regions
journal, September 1993


[130] Glutamine synthetase Escherichia coli
book, January 1970


Three Methods for the Introduction of Foreign DNA into Agrobacterium
book, June 2006


Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L.
journal, November 1989


Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
journal, February 1985


Sequence analysis of a genomic clone encoding a Zc2 protein from Zea mays W64 A
journal, January 1990


Comparison of biosequences
journal, December 1981


Transformation of Zea mays L. Using Agrobacterium tumefaciens and the Shoot Apex
journal, February 1991


Metabolite and light regulation of metabolism in plants: lessons from the study of a single biochemical pathway
journal, May 2001


In Planta Transformation of Tomato
journal, June 2008


Nitrate regulation of metabolism and growth
journal, June 1999


GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
journal, December 1987


Genes responsible for the supervirulence phenotype of Agrobacterium tumefaciens A281.
journal, October 1987


Isolation and characterization of a maize chlorophyll a/b binding protein gene that produces high levels of mRNA in the dark
journal, February 1989


Cloning and characterization of a nodule-enhanced glutamine synthetase-encoding gene from Lupinus luteus
journal, December 1993


Molecular cloning of rat kynurenine aminotransferase: Identity with glutamine transaminase K
journal, October 1994


Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof
patent-application, February 2011


Plants having enhanced nitrogen assimilation/metabolism
patent, July 2000


Structure–function relationships of glutamine synthetases
journal, March 2000


Crystal Structures of Glutamine:Phenylpyruvate Aminotransferase from Thermus thermophilus HB8 INDUCED FIT AND SUBSTRATE RECOGNITION
journal, February 2004


Genes and uses for plant improvement
patent-application, November 2008


Nucleic acids encoding plant glutamine phenylpyruvate transaminase (GPT) and uses thereof
patent-application, July 2010


Gene transfer in plants: Production of transformed plants using Ti plasmid vectors
journal, January 1986


Leaf-specific overexpression of plastidic glutamine synthetase stimulates the growth of transgenic tobacco seedlings
journal, January 2000


Over‐expression of cytosolic glutamine synthetase increases photosynthesis and growth at low nitrogen concentrations
journal, May 2001


[15] Improved vectors for plant transformation: Expression cassette vectors and new selectable markers
journal, January 1987


Lower Dosage Strength Pharmaceutical Compositions Formulated With 3.75% Imiquimod
patent-application, October 2011


Transient and Stable Expression of the Firefly Luciferase Gene in Plant Cells and Transgenic Plants
journal, November 1986


Electroporation: Mechanism and transient expression, stable transformation and biological effects in plant protoplasts
journal, February 1991


Transgenic algae engineered for higher performance
patent, October 2014


Plant glutamine phenylpyruvate transaminase gene and transgenic plants carrying same
patent-application, January 2011


Use of deoxyinosine-containing primers vs degenerate primers for polymerase chain reaction based on ambiguous sequence information
journal, April 1994


Ca lectin gene insertion has the structural features of a transposable element
journal, October 1983


Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997


Enabling technologies for manipulating multiple genes on complex pathways
journal, January 2001


Transient expression from cab-m1 and rbcS-m3 promoter sequences is different in mesophyll and bundle sheath cells in maize leaves.
journal, April 1992


Expression of Ti plasmid genes in monocotyledonous plants infected with Agrobacterium tumefaciens
journal, October 1984


Structure, Evolution, and Regulation of RbcS Genes in Higher Plants
journal, June 1989


Biological functions of proline in morphogenesis and osmotolerance revealed in antisense transgenic Arabidopsis thaliana
journal, April 1999


Genes and uses for plant improvement
patent-application, March 2009


Two Regulatory Genes of the Maize Anthocyanin Pathway Are Homologous: Isolation of B Utilizing R Genomic Sequences
journal, December 1989


Nucleotide sequence of an alfalfa glutamine synthetase gene
journal, May 1986


A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970


Synthesis of 2-oxoglutaramate from L-glutamine
patent-application, February 2003


Method of Conferring Multiple Stress Tolerance and Early Flowering Plants
patent-application, January 2008


Two classes of differentially regulated glutamine synthetase genes are expressed in the soybean nodule: a nodule-specific class and a constitutively expressed class
journal, September 1993


Nitrogen-efficient monocot plants
patent-application, July 2007


Plant Glutamine Phenylpyruvate Transaminase Gene and Transgenic Plants Carrying Same
patent-application, September 2013


The structure and expression of maize genes encoding the major heat shock protein, hsp70
journal, March 1986


Structure, evolution and anaerobic regulation of a nuclear gene encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from maize
journal, August 1989


Agrobacterium-mediated delivery of infectious maize streak virus into maize plants
journal, January 1987


Isolation of Transcriptionally Regulated Root-Specific Genes from Tobacco
journal, July 1990


Cytosolic Glutamine Synthetase in Soybean Is Encoded by a Multigene Family, and the Members Are Regulated in an Organ-Specific and Developmental Manner
journal, January 2002


Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens
journal, January 1987


Transgenic plants with enhanced growth characteristics
patent, January 2018


Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
patent-application, January 2007


Chlorophyllous totipotent cell cultures
patent-application, July 2005


Forcing expression of a soybean root glutamine synthetase gene in tobacco leaves induces a native gene encoding cytosolic enzyme
journal, October 1992


Enzymes having transaminase and aminotransferase activity and methods of use thereof
patent, September 2002


Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
journal, December 1998


Synthese von 1.5-Diaza-cyclooctan-dion-(4.8)-dicarbonsäure-(2.6)
journal, April 1954


Plant Glutamine Phenylpyruvate Transaminase Gene and Transgenic Plants Carrying Same
patent-application, May 2017


The maize GapC4 promoter confers anaerobic reporter gene expression and shows homology to the maize anthocyanin regulatory locus C1
journal, December 1995