Title: Over-expression of a putative oxidoreductase (UcpA) for increasing furfural or 5-hydroxymethylfurfural tolerance

Abstract

The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.

Inventors:

Wang, Xuan; Miller, Elliot N.; Yomano, Lorraine P.; Shanmugam, Keelnatham T.; Ingram, Lonnie O'Neal

Issue Date:

Tue May 24 00:00:00 EDT 2016

Research Org.:

UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED, Gainesville, FL (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1254240

Patent Number(s):

9347077

Application Number:

14/131,468

Assignee:

UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED (Gainesville, FL)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/245 - Escherichia

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/0004 - {Oxidoreductases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P13/001 - {Amines
C12P2203/00 - Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
C12P7/06 - Ethanol, i.e. non-beverage
C12P7/065 - {with microorganisms other than yeasts}
C12P7/10 - substrate containing cellulosic material
C12P7/16 - Butanols
C12P7/18 - polyhydric
C12P7/40 - containing a carboxyl group {including Peroxycarboxylic acids
C12P7/44 - Polycarboxylic acids
C12P7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
C12P7/54 - Acetic acid
C12P7/56 - Lactic acid

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

Y02P20/52 - using catalysts, e.g. selective catalysts

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DOE Contract Number:  

FG36-08GO88142

Resource Type:

Patent

Resource Relation:

Patent File Date: 2012 Jul 09

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 09 BIOMASS FUELS