(Genetic engineering with a gene encoding a soybean storage protein to identify DNA sequences that control its expression): (Final) Progress report, April 1, 1985--March 31, 1988
The goals of the research were to isolate and characterize genomic clones encoding subunits of ..beta..-conglycinin, a soybean seed storage protein. Subsequent to gene isolation the expression of these genes was to be studied via expression in transgenic plants, and the sequences that affect the expression of these genes in transgenic plants was to be identified. An additional goal of the work was to study the chromatin structure of one or more the ..beta..-conglycinin genes. Originally, a single gene (the ..cap alpha..'-subunit gene) was to be studied. In the course of the work, however, we were fortunate to screen a genomic library to find a second member of the gene family, the ..beta..-subunit gene. The identification of this gene adds tremendously to the breadth of the project because, as described previously, its program of gene expression is different than that of the ..cap alpha..'-subunit gene. By using both the ..cap alpha..' and ..beta.. genes we are able to study not only tissue specificity of gene expression, but the differences in temporal regulation of the expression of two members of the gene family. Research is described. 8 refs.
- Research Organization:
- Washington Univ., St. Louis, MO (USA). Dept. of Biology
- DOE Contract Number:
- AC02-81ER10888
- OSTI ID:
- 7187913
- Report Number(s):
- DOE/ER/10888-3; ON: DE88016548
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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