p-aminohippurate transport in the airways: Role of Na sup + and HCO sub 3 -
- Univ. of Connecticut Health Center, Farmington (USA)
The role of Na{sup +} and HCO{sub 3}- in the transport of p-aminohippurate (PAH) across the canine tracheal epithelium was investigated using Ussing chamber techniques and radiolabeled PAH. Under control conditions, net PAH absorption or a tendency toward net PAH absorption was observed. Neither amiloride (10(-4) M), furosemide (10(-3) M), ouabain (2 x 10(-4) M), nor Na+ substitution of the Ringer solution with choline had any effect on unidirectional PAH fluxes. When the Ringer solution was replaced with a HCO{sub 3}(-)-free solution, net PAH absorption was consistently observed. In HCO{sub 3}(-)-free experiments, unidirectional PAH absorptive fluxes were inhibited by mucosal addition of either of the stilbene derivatives, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS, 10(-4) M) or 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS, 10(-4) M). DIDS was more effective than SITS and was also effective in inhibiting PAH absorption in tissues bathed in Ringer solution. Submucosal DIDS or SITS had no effect on PAH fluxes either in HCO{sub 3}(-)-free or Ringer experiments. We conclude that PAH transport in canine tracheal epithelium occurs by a HCO{sub 3}(-)-PAH exchange process located on the luminal membrane. PAH transport is not Na{sup +} dependent but is inhibited by both DIDS and SITS.
- OSTI ID:
- 6983968
- Journal Information:
- American Journal of Physiology; (USA), Vol. 257; ISSN 0002-9513
- Country of Publication:
- United States
- Language:
- English
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MEMBRANE TRANSPORT
SODIUM COMPOUNDS
AMP
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DOGS
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EPITHELIUM
IN VITRO
ISOTOPE DILUTION
MUSCLES
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CELL CONSTITUENTS
DRUGS
ELECTRICAL PROPERTIES
GLYCOSIDES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
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NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
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REACTION KINETICS
RESPIRATORY SYSTEM
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550201* - Biochemistry- Tracer Techniques