Cl sup minus -HCO sup minus sub 3 antiport in rat lacrimal gland
- Univ. of Southern California School of Medicine, Los Angeles (USA)
With the use of analytical subcellular fractionation and tracer uptake methods the authors have demonstrated the presence of a Cl{sup {minus}}-HCO{sup {minus}}{sub 3} antiport mechanism in the rat exorbital lacrimal gland. They find that outwardly directed gradients of HCO{sup {minus}}{sub 3} and of {sup 35}Cl{sup {minus}} accelerated the flux of {sup 36}Cl{sup {minus}} into isolated membrane vesicles. Because vesicle membrane potentials were clamped to 0 mV with K{sup +}-valinomycin, the observed anion gradient-dependent acceleration of Cl{sup {minus}} influx could not be attributed to conductive fluxes. The antiporter had an apparent K{sub 0.5} for Cl{sup {minus}} between 6 and 10 mM. It was sensitive to the stilbene derivatives 4-acetamido-4{prime}-isothiocyanostilbene-2,2{prime}-disulfonic acid (SITS) and 4,4{prime}-diisothiocyanostilbene-2,2{prime}-disulfonic acid (DIDS). It was also sensitive to the loop diuretic furosemide, which has frequently been used in tests for NaKCl{sub 2} symporter activity. Other anions inhibited anion gradient-driven Cl{sup {minus}} influx in the sequence SCN{sup {minus}} > NO{sup {minus}}{sub 3} > Cl{sup {minus}} HCO{sup {minus}}{sub 3} > SO{sup 2{minus}}{sub 4}. The density distribution of Cl{sup {minus}}-HCO{sup {minus}}{sub 3} antiport activity indicated that {approximately}80% of the transporter was associated with intracellular membranes, suggesting the presence of cytoplasmic pools of functional antiporters. Because several studies have already shown the presence of Na{sup +}-H{sup +} antiporter activity in lacrimal acinar cell basolateral membranes, a cellular model for lacrimal acinar electrolyte secretion is proposed in which a parallel array of Cl{sup {minus}}-HCO{sup {minus}}{sub 3} and Na{sup +}-H{sup +} antiporters mediates the Na{sup +}-dependent accumulation of Cl{sup {minus}} against its electrochemical potential gradient.
- OSTI ID:
- 6828630
- Journal Information:
- American Journal of Physiology; (USA), Vol. 255:3; ISSN 0002-9513
- Country of Publication:
- United States
- Language:
- English
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CHLORINE 36
MEMBRANE TRANSPORT
EPITHELIUM
ENZYME ACTIVITY
IONS
SECRETION
SODIUM 22
DIURETICS
LACRIMAL DUCTS
RATS
SULFONIC ACIDS
ALKALI METAL ISOTOPES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
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CHARGED PARTICLES
CHLORINE ISOTOPES
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HEAD
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RADIOISOTOPES
RODENTS
SENSE ORGANS
SODIUM ISOTOPES
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques