Induction of chromosome aberrations in human lymphocytes by in vitro irradiation with. beta. particles from tritiated water
The yields of unstable chromosome aberrations induced in human lymphocytes by ..beta.. particles from tritiated water (HTO) have been measured. HTO was mixed with heparinized blood in various amounts so that doses of 0.25 to 7.0 Gy were delivered in 20 min and 1.5 and 2.5 hr. After culturing for 48 hr, the dicentric yield was measured as a function of dose to the blood and compared with data previously reported for X rays. Using a linear-quadratic dose-effect relation to fit the experimental data, a significant linear contribution was found. The main difference between the coefficients for ..beta.. and X radiation is in the ..cap alpha.. values, indicating that HTO ..beta.. rays are more efficient, particularly at lower doses, in producing two lesions with a single ionizing track. In accord with the theory of dual radiation, the RBE of HTO ..beta.. particles relative to X rays is 2.6 at 0.25 Gy and decreases with increasing dose. When the number of cells without aberrations is plotted against radiation dose, a curve is obtained which is similar in shape to those from human cell survival experiments.
- Research Organization:
- ENEA, CRE, Casaccia, Rome, Italy
- OSTI ID:
- 6847899
- Journal Information:
- Radiat. Res.; (United States), Vol. 97:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BETA PARTICLES
RBE
CHROMOSOMAL ABERRATIONS
RADIOINDUCTION
LYMPHOCYTES
BIOLOGICAL RADIATION EFFECTS
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
IN VITRO
TRITIUM OXIDES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHALCOGENIDES
CHARGED PARTICLES
CONNECTIVE TISSUE CELLS
DATA
INFORMATION
LABELLED COMPOUNDS
LEUKOCYTES
MATERIALS
MUTATIONS
NUMERICAL DATA
OXIDES
OXYGEN COMPOUNDS
RADIATION EFFECTS
SOMATIC CELLS
TRITIUM COMPOUNDS
560121* - Radiation Effects on Cells- External Source- (-1987)
550301 - Cytology- Tracer Techniques