Hydrolysis of wheat straw hemicellulose with trifluoroacetic acid. Fermentation of xylose with Pachysolen tannophilus
Treatment of wheat straw 1N trifluoroacetic acid (TFA) for 7 h at reflux temperature yielded 23% xylose based upon initial straw weight. This corresponds to about an 80% xylose yield based on the xylan content of the hemicellulose. The cellulose component of wheat straw was largely unaffected, as evidenced by low glucose yields. Decomposition of xylose by prolonged refluxing (23h) was minimal in 1N TFA compared to 1N HCl. Treatment of wheat straw with refluxing 1N TFA converts about 10% of the lignin initially present in straw into water-soluble lignin fragments. Fermentation of the xylose-rich wheat straw hydrolyzate to ethanol with Pachysolen tannophilus was comparable to the fermentation of reagent grade xylose, indicating that furfural and toxic lignin by-products were not produced by 1N TFA in sufficient amounts to impair cell growth and ethanol production. Cellulase treatment of the wheat straw residue after TFA hydrolysis resulted in a 70-75% conversion of the cellulose into glucose.
- Research Organization:
- Dept. of Agriculture, Peoria, IL
- OSTI ID:
- 6416274
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 26:9
- Country of Publication:
- United States
- Language:
- English
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Fermentation of wheat straw hemicelluloses to ethanol by Pachysolen tannophilus
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Related Subjects
ETHANOL
BIOSYNTHESIS
HEMICELLULOSE
ACID HYDROLYSIS
XYLOSE
FERMENTATION
EXPERIMENTAL DATA
STRAW
WHEAT
YEASTS
ALCOHOLS
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
CEREALS
CHEMICAL REACTIONS
DATA
DECOMPOSITION
FUNGI
GRASS
HYDROLYSIS
HYDROXY COMPOUNDS
INFORMATION
LYSIS
MICROORGANISMS
MONOSACCHARIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PENTOSES
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)