Production and properties of extracellular endoxylanase from Neurospora crassa
Neurospora crassa 870 produced 14 and 0.025 U of extracellular xylanase (1,4-..beta..-o-xylan xylanohydrolase; EC 3.2.1.8) and 4b-xylosidase (1,4-4b-xylan xylohydrolase; EC 3.2.1.37) per ml, respectively, in 4 days when commercial xylan was used as a carbon source. The effects of pH and carbon sources on xylanase production by N. crassa are discussed. Two xylanases (I and II) were purified and had pI values of 4.8 and 4.5 and molecular weights of 33,000 and 30,000. The maximum degree of hydrolysis of xylan by the extracellular culture broth was 66% in 4 h. The end products of xylan hydrolysis by xylanase I and II showed the presence of xylose, xylobiose, xylotriose, xylotetraose, xylopentose, and arabinose, indicating that they are endoxylanases capable of hydrolyzing 1,3-..cap alpha..-L-arabinofuranosyl branch points. Both xylanases showed activity toward carboxymethyl cellulose but no activity toward para-nitrophenyl-..beta..-dxyloside or laminarin. Xylanase I showed appreciable activity toward para-nitrophenyl-..beta..-D-glucoside, whereas xylanase II was inactive.
- Research Organization:
- National Chemical Lab., Poona, India
- OSTI ID:
- 6147563
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 48:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
NEUROSPORA
CELLULOLYTIC ACTIVITY
ENZYME ACTIVITY
XYLANASE
BIOSYNTHESIS
XYLANS
ENZYMATIC HYDROLYSIS
PURIFICATION
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HEMICELLULOSE
HYDROLASES
HYDROLYSIS
LYSIS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
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