Production and properties of xylan-degrading enzymes from Cellulomonas uda

Journal Article · Tue Apr 01 00:00:00 EST 1986 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5328232

Rapp, P; Wagner, F

Xylan degradation and production of ..beta..-xylanase and ..beta..-xylosidase activities were studied in cultures of Cellulomonas uda grown on purified xylan from birchwood. Beta-xylanase activity was found to be associated with the cells, although in various degrees. The formation of ..beta..-xylanase activity was induced by xylotriose and repressed by xylose. Beta-xylosidase activity was cell bound. Both constitutive and inducible ..beta..-xylosidase activities were suggested. Beta-xylanase and ..beta..-xylosidase activities were inhibited competitively be xylose. Beta-xylanase activity had a pronounced optimum pH of 5.8, whereas the optimum pH of ..beta..-xylosidase activity ranged from 5.4 to 6.1. The major products of xylan degradation by a crude preparation of ..beta..-xylanase activity, in decreasing order of amount, were xylobiose, xylotriose, xylose and small amounts of xylotetraose. This pattern suggests that ..beta..-xylanase activity secreted by C. uda is of the endosplitting type. Supernatants of cultures grown on cellulose showed not only ..beta..-glucanase byt also ..beta..-xylanase activity. The latter could be attributed to an endo-1,4-..beta..-glucanase activity which had a low ..beta..-xylanase activity. 54 references.

• Research Organization: Universitaet Bransschweig, D-3300 Brunswick, Germany, F.R.
• OSTI ID: 5328232
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 51:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
BACTERIA
ENZYME ACTIVITY
XYLANS
BIODEGRADATION
CELLULOSE
CULTURE MEDIA
GROWTH
INHIBITION
OPTIMIZATION
PH VALUE
XYLANASE
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
GLYCOSYL HYDROLASES
HEMICELLULOSE
HYDROLASES
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
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