The existence of 25-hydroxyvitamin D sub 3 -1. alpha. -hydroxylase in the liver of carp and bastard halibut

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(91)90355-F· OSTI ID:5922605

Takeuchi, Atsuko; Okano, Toshio; Kobayashi, Tadashi ^[1]

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1. Kobe Women's College of Pharmacy (Japan)

We have found that carp and bastard halibut contain 25-hydroxyvitamin D{sub 3}(25-D{sub 3})-1{alpha}-hydroxylase in the liver besides in the kidney by the following in vivo and in vitro experiments. When ({sup 3}H)-25-D{sub 3} was intraperitoneally injected to vitamin D(D)-deficient carp and normal bastard halibut, the profiles of high-performance liquid chromatography (HPLC) of the plasma lipid extract showed the formation of a peak corresponding to ({sup 3}H)-1{alpha},25-dihydroxyvitamin D{sub 3}(1,25-D{sub 3}). When ({sup 3}H)25-D{sub 3} was incubated with liver homogenates of the fish, a peak corresponding to ({sup 3}H)-1,25-D{sub 3} was also observed in the profile of HPLC. The formation of the metabolite was confirmed by the thermal isomerization into the pre-isomer and mass fragmentography. Although the 1{alpha}-hydroxylase was also observed in the kidney, the activity of the enzyme was lower than that in the liver. The results suggest that 25-D{sub 3}-1{alpha}-hydroxylase exists in the liver of carp and bastard halibut and the 25-D{sub 3} formed from D{sub 3} in the liver is immediately metabolized into 1,25-D{sub 3} in the same tissue.

• OSTI ID: 5922605
• Journal Information: Life Sciences; (USA), Vol. 48:3; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HYDROXYLASES
ENZYME ACTIVITY
VITAMIN D
METABOLISM
FISHES
IN VITRO
IN VIVO
KIDNEYS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AQUATIC ORGANISMS
BODY
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ORGANS
OXIDOREDUCTASES
SEPARATION PROCESSES
VERTEBRATES
VITAMINS
550201* - Biochemistry- Tracer Techniques