Stimulation of 25-hydroxyvitamin D/sub 3/-1. cap alpha. -hydroxylase by estrogen

Journal Article · Sat Jan 01 00:00:00 EST 1977 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(77)90105-9· OSTI ID:7076061

Castillo, L; Tanaka, Y; DeLuca, H F; Sunde, M L

Homogenates of kidney from laying Japanese quail incubated in vitro with 25-hydroxy-(26,27-/sup 3/H)vitamin D/sub 3/ produce more 1,25-dihydroxy-(26,27-/sup 3/H)vitamin D/sub 3/ than do homogenates of kidney from mature nonlaying females or males maintained on the same diet and under identical conditions. Instead, the homogenates from male quail or nonlaying female quail convert 25-hydroxyvitamin D/sub 3/ to 24,25-dihydroxyvitamin D/sub 3/. The administration of 5 mg of estradiol to mature male quail 24 h prior to sacrifice suppressed the 25-hydroxyvitamin D/sub 3/-24-hydroxylase and markedly stimulated 25-hydroxyvitamin D/sub 3/-1-hydroxylase. The administration of estradiol to male quail caused hypercalcemia, which responded more slowly than did the 1-hydroxylase. As little as 0.1 mg of estradiol/quail was found effective in stimulating the 1-hydroxylase and suppressing the 24-hydroxylase. Other hormones such as follicle stimulating hormone (FSH), cortisone, testosterone, and progesterone, even at high dose levels, produced little or no change in the 25-hydroxyvitamin D/sub 3/-1-hydroxylase. Testosterone did, however, suppress the 25-hydroxyvitamin D/sub 3/-24-hydroxylase. The stimulation of the 25-hydroxyvitamin D/sub 3/-1-hydroxylase by parathyroid hormone was of a smaller magnitude than that of the estradiol, and the effects of the two hormones were additive, suggesting that they function by a different mechanism.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 7076061
• Journal Information: Arch. Biochem. Biophys.; (United States), Vol. 179
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM
METABOLISM
ENZYMES
STIMULATION
ESTROGENS
BIOCHEMISTRY
BIRDS
CHOLECALCIFEROL
CORTISONE
FSH
HORMONES
HYDROXYLASE
INHIBITION
KIDNEYS
PARATHORMONE
PARATHYROID GLANDS
PROGESTERONE
PROTEINS
SEX DEPENDENCE
TESTOSTERONE
ADRENAL HORMONES
ALKALINE EARTH METALS
ANDROGENS
ANDROSTANES
ANIMALS
BODY
CHEMISTRY
CORTICOSTEROIDS
ELEMENTS
ENDOCRINE GLANDS
GLANDS
GLUCOCORTICOIDS
GONADOTROPINS
HYDROXY COMPOUNDS
KETONES
METALS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PITUITARY HORMONES
PREGNANES
STEROID HORMONES
STEROIDS
VERTEBRATES
VITAMIN D
VITAMINS
550500* - Metabolism
550200 - Biochemistry