Autoradiographic localization and characterization of angiotensin II binding sites in the spleen of rats and mice

Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Peptides (Fayetteville, N.Y.); (United States)

DOI:https://doi.org/10.1016/0196-9781(87)90050-7· OSTI ID:5905125

Castren, E; Kurihara, M; Saavedra, J M

Specific binding sites for angiotensin II (Ang II) were localized in the red pulp of the spleen of rats and mice by quantitative autoradiography using /sup 125/I-Sar1-Ang II as a ligand. In the rat, the binding was saturable and specific, and the rank order for Ang II derivatives as competitors of /sup 125/I-Sar1-Ang II binding correlates well with their affinity for Ang II receptors in other tissues. Kinetic analysis in the rat spleen revealed a single class of binding sites with a KD of 1.11 nM and a Bmax value of 81.6 fmol/mg protein. Ang II binding sites were also localized on isolated rat spleen cells with similar affinity but with much lower Bmax, 9.75 fmol/mg protein. Ang II receptors were not detected in thymus sections from rats or mice, or on isolated rat thymocytes. The binding sites described here might represent a functional Ang II receptor with a role in the regulation of splenic volume and blood flow and in the modulation of the lymphocyte function.

• Research Organization: National Institute of Mental Health, Bethesda, MD
• OSTI ID: 5905125
• Journal Information: Peptides (Fayetteville, N.Y.); (United States), Vol. 8:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANGIOTENSIN
RECEPTORS
BIOLOGICAL LOCALIZATION
CHEMICAL COMPOSITION
AFFINITY
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
IODINE 125
LIGANDS
MICE
RATS
SPLEEN
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
VASOCONSTRICTORS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques