Localization of angiotensin II receptors in ovarian follicles and the identification of angiotensin II in rat ovaries

Journal Article · Wed Apr 01 00:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.8.2489· OSTI ID:6025233

Husain, A; Bumpus, F M; De Silva, P; Speth, R C

Specific, high-affinity (K/sub d/ approx. = 0.6 nM), and saturable (3.3 fmol/mg of tissue, wet weight) binding of /sup 125/I-labeled (Sar/sup 1/,Ile/sup 8/)angiotensin II to rat ovarian membranes was observed. Displacement of /sup 125/I-labeled (Sar/sup 1/,Ile/sup 8/)angiotensin II binding to rat ovarian membranes by angiotensin II analogs and fragments resembled the potency order of these compounds on angiotensin II receptors in other tissues. Several unrelated peptides, including follicle-stimulating hormone at 10..mu..M, did not displace ovarian /sup 125/I-labeled (Sar/sup 1/, Ile/sup 8/)angiotensin II binding. Autoradiograms of /sup 125/I-labeled (Sar/sup 1/,Ile/sup 8/)angiotensin II binding to ovarian sections indicated that the angiotensin II receptor binding sites were localized exclusively to a subpopulation of follicles, occurring on the granulosa and theca interna cells. Other follicles were devoid of /sup 125/I-labeled (Sar/sup 1/,Ile/sup 8/)angiotensin II binding sites. Angiotensin II immunoreactive material was also identified in the ovary. The concentration of ovarian Ang II immunoreactivity was 8- to 75-fold greater than that of plasma, was not reduced in bilaterally nephrectomized rats, and was shown by high-pressure liquid chromatographic analysis to be the native angiotensin II octapeptide. The presence of angiotensin II and its receptor binding sites in the ovary suggests a role for angiotensin II as a regulator of ovarian function.

• Research Organization: Research Institute of the Cleveland Clinic Foundation, Cleveland, OH
• OSTI ID: 6025233
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:8
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
ANGIOTENSIN
TISSUE DISTRIBUTION
OVARIES
AUTORADIOGRAPHY
RECEPTORS
CELL MEMBRANES
IODINE 125
LIQUID COLUMN CHROMATOGRAPHY
RATS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CHROMATOGRAPHY
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DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FEMALE GENITALS
GLOBULINS
GONADS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
VASOCONSTRICTORS
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