(Starch synthesis in the maize endosperm as affected by starch synthesizing mutants)
A recent findings is that the activity of the solubilized starch granule-bound enzyme is enhanced by the addition of Glc-1,6-bisP to a reaction mixture containing Glc-1-P as a substrate. The addition of F-1,6-bisP is nearly as effective as that of Glc-1,6-bisP while F-1-P, F-6-P, R-1-P, R-5-P, Ru-1,5-bisP, or Maltose-1-P are ineffective. It is a tenable hypothesis at the present time that Glc-1,6-bisP is also utilized as a substrate by the oligosaccharide synthase or another enzyme associated with it in the high molecular complex which contains the oligosaccharide synthase that has been freed from the starch granules by the procedure of MacDonald and Preiss. This fraction also appears to be capable of interconverting Glc-1,6-bisP and F-1,6-bisP and this can account for the observation that F-1,6-bisP has the ability to accelerate the incorporation of Glc-1-P into oligosaccharides as does Glc-1,6-bisP. They are attempting currently to test the possibility that Glc-1,6-P is utilized to some extent as a substrate and that the PO{sub 4} present on C6 in some Glc moieties in the oligosaccharide chains derives from this source.
- Research Organization:
- Wisconsin Univ., Madison, WI (USA). Lab. of Genetics
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- AC02-82ER12031
- OSTI ID:
- 5859904
- Report Number(s):
- DOE/ER/12031-T3; ON: DE91010194
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MAIZE
BIOCHEMISTRY
OLIGOSACCHARIDES
BIOSYNTHESIS
ENZYME ACTIVITY
GENES
GLUCOSE
LIGASES
MUTANTS
PHOSPHATES
ALDEHYDES
CARBOHYDRATES
CEREALS
CHEMISTRY
ENZYMES
GRASS
HEXOSES
LILIOPSIDA
MAGNOLIOPHYTA
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PLANTS
SACCHARIDES
SYNTHESIS
550200* - Biochemistry