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Title: Proteome and phosphoproteome analysis of starch granule-associated proteins from normal maize and mutants affected in starch biosynthesis

Journal Article · · Journal of Experimental Botany
DOI:https://doi.org/10.1093/jxb/ern198· OSTI ID:1625356
 [1];  [1];  [2];  [2];  [1]
  1. Institut National de la Recherche Agronomique, Nantes Cedex (France). Unite de Recherche Biopolymeres, Interactions, Assemblages
  2. Iowa State Univ., Ames, IA (United States). Dept. of Biochemistry, Biophysics and Molecular Biology
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In addition to the exclusively granule-bound starch synthase GBSSI, starch granules also bind significant proportions of other starch biosynthetic enzymes, particularly starch synthases (SS) SSI and SSIIa, and starch branching enzyme (BE) BEIIb. Whether this association is a functional aspect of starch biosynthesis, or results from non-specific entrapment during amylopectin crystallization, is not known. This study utilized genetic, immunological, and proteomic approaches to investigate comprehensively the proteome and phosphoproteome of Zea mays endosperm starch granules. SSIII, BEI, BEIIa, and starch phosphorylase were identified as internal granule-associated proteins in maize endosperm, along with the previously identified proteins GBSS, SSI, SSIIa, and BEIIb. Genetic analyses revealed three instances in which granule association of one protein is affected by the absence of another biosynthetic enzyme. First, eliminating SSIIa caused reduced granule association of SSI and BEIIb, without affecting GBSS abundance. Second, eliminating SSIII caused the appearance of two distinct electrophoretic mobility forms of BEIIb, whereas only a single migration form of BEIIb was observed in wild type or any other mutant granules examined. Third, eliminating BEIIb caused significant increases in the abundance of BEI, BEIIa, SSIII, and starch phosphorylase in the granule, without affecting SSI or SSIIa. Analysis of the granule phosphoproteome with a phosphorylation-specific dye indicated that GBSS, BEIIb, and starch phosphorylase are all phosphorylated as they occur in the granule. These results suggest the possibility that starch metabolic enzymes located in granules are regulated by posttranslational modification and/or protein–protein interactions

Research Organization:
Iowa State Univ., Ames, IA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
FG02-05ER15706
OSTI ID:
1625356
Journal Information:
Journal of Experimental Botany, Vol. 59, Issue 12; ISSN 0022-0957
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
United States
Language:
English

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Deficiency of maize starch-branching enzyme i results in altered starch fine structure, decreased digestibility and reduced coleoptile growth during germination journal January 2011
Starch synthase 4 is located in the thylakoid membrane and interacts with plastoglobule-associated proteins in Arabidopsis journal September 2014
Development of high amylose wheat through TILLING journal January 2012
In vivo phosphoproteome characterization reveals key starch granule-binding phosphoproteins involved in wheat water-deficit response journal October 2017
Post-translational Modifications in Regulation of Chloroplast Function: Recent Advances journal February 2017
Self-preserving lognormal volume-size distributions of starch granules in developing sweetpotatoes and modulation of their scale parameters by a starch synthase II (SSII) journal October 2016
Protein Kinases and Phosphatases of the Plastid and Their Potential Role in Starch Metabolism journal July 2018
Cornstarch is less allergenic than corn flour in dogs and cats previously sensitized to corn journal June 2018
Proteomic Analysis of Starch Biosynthesis in Maize Seeds journal July 2019
Maize proteomics: An insight into the biology of an important cereal crop journal January 2013
Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology journal September 2019
Quantitative phosphoproteomic analysis of early seed development in rice (Oryza sativa L.) journal November 2015
Non-GMO potato lines with an altered starch biosynthesis pathway confer increased-amylose and resistant starch properties: Non-GMO potato lines with altered starch biosynthesis journal February 2017
Recreating the synthesis of starch granules in yeast journal November 2016
Allelic effects on starch structure and properties of six starch biosynthetic genes in a rice recombinant inbred line population journal March 2015
Proteomic Dissection of Endosperm Starch Granule Associated Proteins Reveals a Network Coordinating Starch Biosynthesis and Amino Acid Metabolism and Glycolysis in Rice Endosperms journal May 2016
Starch Biosynthesis in Relation to Resistant Starch book September 2013
Allelic variants of the amylose extender mutation of maize demonstrate phenotypic variation in starch structure resulting from modified protein-protein interactions journal November 2011
Starch Biosynthesis in Relation to Resistant Starch book September 2013
Protein-Protein Interactions During Starch Biosynthesis book January 2015
Genetic and genomic analysis of the seed-filling process in maize based on a logistic model journal July 2019
Dynamic development of starch granules and the regulation of starch biosynthesis in Brachypodium distachyon: comparison with common wheat and Aegilops peregrina journal August 2014
Increasing the amylose content of durum wheat through silencing of the SBEIIagenes journal July 2010
Formation of starch in plant cells journal May 2016
Distinct metabolic changes between wheat embryo and endosperm during grain development revealed by 2D-DIGE-based integrative proteome analysis journal April 2016
PROTEIN TARGETING TO STARCH Is Required for Localising GRANULE-BOUND STARCH SYNTHASE to Starch Granules and for Normal Amylose Synthesis in Arabidopsis text January 2015
The primitive rhodophyte Cyanidioschyzon merolae contains a semiamylopectin-type, but not an amylose-type,  -glucan journal April 2010
PROTEIN TARGETING TO STARCH Is Required for Localising GRANULE-BOUND STARCH SYNTHASE to Starch Granules and for Normal Amylose Synthesis in Arabidopsis journal February 2015
Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.) journal January 2012
The cereal starch endosperm development and its relationship with other endosperm tissues and embryo journal August 2014
Waxy allele diversification in foxtail millet (Setaria italica) landraces of Taiwan journal December 2018
Starch as a source, starch as a sink: the bifunctional role of starch in carbon allocation journal July 2017
iTRAQ-based quantitative proteome and phosphoprotein characterization reveals the central metabolism changes involved in wheat grain development journal January 2014
A rapid and universal method for isolating starch granules in plant tissues journal August 2019
Preferential binding of SBD from Arabidopsis thaliana SSIII to polysaccharides: Study of amino acid residues involved journal March 2011
Starch formation inside plastids of higher plants journal May 2018
Proteome Profile of Starch Granules Purified from Rice (Oryza sativa) Endosperm journal December 2016

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
Plant Sciences
Protein phosphorylation
proteomics
starch biosynthesis
starch granules
Zea mays