The effect of ozone exposure on rat alveolar macrophage arachidonic acid metabolism

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Experimental Lung Research; (USA)

DOI:https://doi.org/10.3109/01902149109063281· OSTI ID:5797315

Madden, M C; Eling, T E; Dailey, L A; Friedman, M ^[1]

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1. Univ. of North Carolina, Chapel Hill (USA)

Rat alveolar macrophages were prelabeled with {sup 3}H-arachidonic acid ({sup 3}H-AA) and exposed to air or O3 (0.1-1.0 ppm) in vitro for 2 h. Alveolar macrophages released 3.6-fold more tritium at the 1.0 ppm exposure concentration compared with air-exposed macrophages. A significantly increased production of several {sup 3}H-AA metabolites, including 6-keto-PGF1 alpha, thromboxane B2, 12-hydroxy-5,8,10-heptadecatrienoic acid, prostaglandins E2 and D2, leukotrienes B4 and D4, and 15-hydroxyeicosatetraenoic acid was formed by macrophages exposed to 1.0 ppm O3 compared with air-exposed macrophages as determined by high performance liquid chromatography (HPLC) analysis. O3 exposure did not alter macrophage {sup 3}H-AA metabolism in response to calcium ionophore A23187. The largest tritiated peak observed in the HPLC chromatograms of O{sub 3}-exposed cells was a polar complex of products that contained various phospholipids and neutral lipids (including diacylglycerol) and possibly degradation products of {sup 3}H-AA and some of its metabolites. These changes in macrophage arachidonic acid metabolism may play an important role in the lung response to O{sub 3} exposure in vivo.

• OSTI ID: 5797315
• Journal Information: Experimental Lung Research; (USA), Vol. 17:1; ISSN 0190-2148
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
METABOLISM
OZONE
TOXICITY
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
MACROPHAGES
METABOLITES
PROSTAGLANDINS
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques