Arachidonic and eicosapentaenoic acid metabolism in bovine neutrophils and platelets: effect of calcium ionophore
Substitution of dietary fatty acids has potential for altering the inflammatory response. The purpose of the present study was to define the metabolites of arachidonic acid (AA) and eicosapentaenoic acid (EPA) secreted by bovine peripheral blood neutrophils and platelets. High performance liquid chromatography was used to characterize cyclooxygenase and lipoxygenase metabolites secreted in response to the calcium ionophore A23187. Cells were prelabelled with /sup 3/H-AA or /sup 3/H-EPA prior to challenge with the calcium ionophore. Bovine neutrophils secreted leukotriene B4 (LTB4) and 5-hydroxyeicosatetraenoic acid (5-HETE) as the major metabolites of AA, as well as the corresponding leukotriene B5 (LTB5) and 5-hydroxyeicosapentaenoic acid (5-HEPE) metabolites of EPA. Peptidoleukotrienes derived from /sup 3/H-AA or /sup 3/H-EPA were not detected under these conditions. The major tritiated metabolites secreted from bovine platelets were: thromboxane A2, measured as the stable metabolite thromboxane B2 (TXB2); hydroxyheptadecatrienoic acid (HHT) and 12-HETE derived from /sup 3/H-AA; and the omega-3 analogs TXB3 and 12-HEPE, derived from /sup 3/H-EPA. Preferred substrate specificities existed amongst the AA- and EPA-derived metabolites for the intermediary enzymes involved in the arachidonic acid cascade. These findings support the hypothesis that substitution of membrane-bound AA by EPA has potential for modulation of the host inflammatory response following cellular phospholipid mobilization.
- Research Organization:
- Washington State Univ., Pullman
- OSTI ID:
- 6090072
- Journal Information:
- J. Leukocyte Biol.; (United States), Vol. 42:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
The effects of the oral administration of fish oil concentrate on the release and the metabolism of (/sup 14/C)arachidonic acid and (/sup 14/C)eicosapentaenoic acid by human platelets
Production of metabolic products of arachidonic acid during cell-cell interactions
Related Subjects
ARACHIDONIC ACID
METABOLISM
CALCIUM
BIOLOGICAL FUNCTIONS
EICOSANOIC ACID
METABOLITES
SECRETION
BLOOD PLATELETS
CATTLE
LIQUID COLUMN CHROMATOGRAPHY
NEUTROPHILS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALINE EARTH METALS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DOMESTIC ANIMALS
ELEMENTS
FUNCTIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
METALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RUMINANTS
SEPARATION PROCESSES
VERTEBRATES
550501* - Metabolism- Tracer Techniques