Radioimmunoassay of serum parathyrin: comparison of five commercial kits

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5639395

Gorog, R H ^[1]; Hakim, M K; Thompson, N W; Rigg, G A; McCann, D S

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1. Wayne County General Hospital, Wayne, MI

We compared serum parathyrin radioimmunoassay data obtained with five commercially available kits for normal subjects, patients on dialysis, and patients with hyperparathyroidism. The Cambridge Nuclear kit gave low results, did not distinguish the dialysis-group sera from the normal sera, and had the greatest CV (56%) both inter- and intra-assay. The Immuno Nuclear PTH-II kit gave more satisfactory clinical data than their PTH-C-terminal kit; however, as with most procedures involving a substantial and variable nonspecific binding correction for each serum, parallelism between samples andthe standard curve was poor, turnaround time slow, and inter-assay CV high (39%). Unlike the other kits, the Nichols and the Iso-Tex products depend on a direct methodology; they displayed good parallelism and distinguished among our three populations. A Scatchard plot of results with the Nichols kit was closest to the ideal linear model; the Iso-Tex had the shortest turnaround time.

• OSTI ID: 5639395
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 28:1
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ENDOCRINE DISEASES
DIAGNOSIS
PARATHORMONE
RADIOIMMUNOASSAY
BLOOD SERUM
COMPARATIVE EVALUATIONS
DIALYSIS
EVALUATED DATA
MEASURING METHODS
PATIENTS
DATA
DISEASES
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
NUMERICAL DATA
PEPTIDE HORMONES
RADIOASSAY
SEPARATION PROCESSES
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics