Development and validation of a new radioimmunoassay for parathyrin (PTH)

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5545367

Kao, P C; Jiang, N S; Klee, G G; Purnell, D C

A radioimmunoassay for human parathyrin has been developed and characterized with whole-molecule (residues 1-84) human parathyrin and with the 1-34, 44-68,and 53-84 amino acid residue fragments of it. The antiserum used reacted with the whole molecule and with the 44-68 and 53-84 fragments, but not with the 1-34 fragment. Parathyrin concentrations in the serum of 118 normal subjects and of 112 patients with surgically proved primary hyperparathyroidism were determined with this assay. The mean results were 39 (SD 13) ..mu..L-Eq/mL for the normals and 111 (SD 77) ..mu..L-Eq/mL for the patients with primary hyperparathyroidism (p < 0.0005). The upper 95% confidence limit of the normal range was 60 ..mu..L-Eq/mL. For 54 patients with primary hyperparathyroidism, the preoperative values for serum parathyrin, calcium, and phosphate-but not creatinine-were statistically different from the postoperative values (paired t-test, p <0.0005). Normal subjects showed significant (p <0.0005) differences in serum calcium concentrations but not in parathyrin concentrations, compared with concentrations found in cancer patients and patients who had thiazide induced hypercalcemia. Phosphate concentration in serum, although not a specific indicator of disease, is a valuable clue to the diagnosis of primary hyperparathyroidism.

• Research Organization: Mayo Clinic and Mayo Foundation, Rochester, MN
• OSTI ID: 5545367
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 28:1
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ENDOCRINE DISEASES
DIAGNOSIS
PARATHORMONE
RADIOIMMUNOASSAY
BLOOD SERUM
CALCIUM
COMPARATIVE EVALUATIONS
IODINE COMPOUNDS
MEASURING METHODS
PATIENTS
PHOSPHATES
QUANTITY RATIO
SEPARATION PROCESSES
UPTAKE
ALKALINE EARTH METALS
DISEASES
ELEMENTS
HALOGEN COMPOUNDS
HORMONES
ISOTOPE APPLICATIONS
METALS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS COMPOUNDS
RADIOASSAY
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics