Imaging of human leukemic T-cell xenografts in nude mice by radiolabeled monoclonal antibodies and F(ab')2 fragments
Monoclonal antibodies (MoAb) that react with the T-lymphocyte markers called cluster of differentiation CD5 and CD2 were labeled with iodine 131 (/sup 131/I) and were injected intravenously in nude mice bearing solid subcutaneous xenografts derived from the human T-cell leukemia line Ichikawa. Both MoAb anti-CD5 and anti-CD2 yielded favorable mean tumor to whole-body ratios of 3.8 and 5.1, respectively. These ratios were further increased up to 10.0 for MoAb anti-CD5 and 15.5 for MoAb anti-CD2 by using their F(ab')2 fragments. The tumors could be imaged clearly by external scanning after injection of F(ab')2 fragments from both MoAb. F(ab')2 fragments from MoAb anti-CD2 and of a third MoAb recognizing the clonotypic determinant (Ti) of the antigen receptor expressed by the human T-cell line Jurkat were injected in mice bearing intrasplenic Jurkat xenografts. A selective localization of both fragments in tumor tissue was demonstrated with mean tumor to whole-body ratios of 7.5 and 4.1 for MoAb anti-CD2 and anti-Ti, respectively. These in vivo experimental results may provide useful information for the potential use of radiolabeled MoAb and fragments in the diagnosis and treatment of patients with T-cell lymphoma and different other forms of T-cell malignancies.
- Research Organization:
- Univ. of Lausanne, Epalinges, Switzerland
- OSTI ID:
- 5513495
- Journal Information:
- Cancer (Philadelphia); (United States), Vol. 61:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LYMPHOCYTES
CELL DIFFERENTIATION
MONOCLONAL ANTIBODIES
DIAGNOSTIC USES
TUMOR CELLS
TRANSPLANTS
INTRAVENOUS INJECTION
IODINE 131
LEUKEMIA
MICE
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
HEMIC DISEASES
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RODENTS
SOMATIC CELLS
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics