Localization of human osteogenic sarcoma xenografts in nude mice by a monoclonal antibody labeled with radioiodine and indium-111

Journal Article · Sun Mar 01 00:00:00 EST 1987 · J. Nucl. Med.; (United States)

OSTI ID:6595778

Sakahara, H; Endo, K; Nakashima, T; Koizumi, M; Kunimatsu, M; Kawamura, Y; Ohta, H; Nakamura, T; Tanaka, H; Kotoura, Y

Monoclonal antibody OST7 reacts specifically with human osteogenic sarcoma. Whole immunoglobulin G (IgG) and the F(ab')2 fragment of OST7 were labeled with radioiodine and indium-111 (/sup 111/I), and injected into athymic nude mice bearing xenografts of human osteogenic sarcoma (KT005). All radiolabels retained their antigen-binding activities allowing clear visualization of transplanted tumors. Tissue distribution, however, varied. The net tumor concentration of /sup 111/In-lableled whole IgG was higher and was retained longer than that of radioiodinated one, but /sup 111/In-labeled antibody showed a lower tumor-to-liver and tumor-to-kidney ratio. In radioiodinated OST7, F(ab')2 fragments provided much better images with much higher tumor-to-tissue ratios than whole IgG. Using F(ab')2 fragments as a carrier for /sup 111/In, little image improvement was obtained due to the high radioactivity in the liver and kidney. The different distributions among these radiolabels suggest that the choice between intact IgG and F(ab')2 fragments and between radioiodine and /sup 111/In depends on the intended use.

• Research Organization: Kyoto Univ., Japan
• OSTI ID: 6595778
• Journal Information: J. Nucl. Med.; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
INDIUM 111
TISSUE DISTRIBUTION
MONOCLONAL ANTIBODIES
BIOLOGICAL LOCALIZATION
DIAGNOSTIC USES
OSTEOSARCOMAS
DIAGNOSIS
TRANSPLANTS
IMMUNOGLOBULINS
KIDNEYS
LIVER
MAN
MICE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
GLOBULINS
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPES
RODENTS
SARCOMAS
SKELETAL DISEASES
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics