Intrinsic and extrinsic zinc absorption by infants from cow milk formula
Zinc 67 was injected into a cow, and the enriched milk was used to prepare a formula intrinsically labelled with /sup 67/Zn and with total zinc concentration near 2.5 mg/kg. Extrinsic label (85.03 atom percent /sup 70/Zn) was added (0.10 mg Zn/kg) as the chloride to the formula 12-24 hours before feeding. Metabolic balance studies of 72 hours duration were performed with five normal infants 152 to 397 days of age. Enriched formula was fed during the first 24 hours, followed by unenriched formula for the remainder of the time. Isotope ratios of /sup 70/Zn referenced to /sup 68/Zn were determined on formula and fecal samples by inductively-coupled plasma/mass spectrometry of zinc extracts with relative standard deviations near 1%. Fecal enrichment of one infant did not return to baseline by the end of the balance period. For the remaining four infants zinc retention was negative, with total zinc intake between 6.8 and 8.7 mg/72 hours and total zinc excretion between 6.9 and 11.2 mg/72 hours. Zinc absorption based on balance data ranged from -26 to +12% of intake. Fractional absorption (Mean +/- S. D.) of /sup 67/Zn was 20.2 +/- 12.0% and that of /sup 70/Zn was 21.7 +/- 11.3%. The difference was not statistically significant by Student's t test for paired data. It seems that the extrinsic zinc labels were absorbed similarly.
- Research Organization:
- Iowa State Univ., Ames, IA
- OSTI ID:
- 5461758
- Report Number(s):
- CONF-8604222-; TRN: 86-028421
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ZINC
INTESTINAL ABSORPTION
METABOLISM
CATTLE
FECES
MILK
RETENTION
TRACER TECHNIQUES
ZINC 67
ZINC 68
ZINC 70
ABSORPTION
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
DOMESTIC ANIMALS
ELEMENTS
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
FOOD
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
METALS
NUCLEI
RUMINANTS
STABLE ISOTOPES
UPTAKE
VERTEBRATES
WASTES
ZINC ISOTOPES
550501* - Metabolism- Tracer Techniques