Analysis of stable isotopes in urine to determine the fractional absorption (FA) of zinc

Conference · Fri Mar 15 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5146503

Friel, J; Naake, V Jr; Miller, L; Fennessey, D; Hambidge, K ^[1]

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1. Univ. of Colorado, Denver (United States) Memorial Univ., St. John's, Newfoundland (Canada)

{sup 68}Zn and {sup 70}Zn were administered orally or intravenously to 4 normal adults to determine fractional absorption (FA) of zinc. Urine and fecal samples were collected for 7 days after giving the isotopes. Feces were dried, ashed and passed through AG-1 resin columns to separate zinc from other trace elements. Urine was evaporated, ashed and passed through Chelex-100 resin to separate Ca, Mg, P, and K before passing eluent through AG-1 resin. All samples were analyzed by Fast Atom Bombardment Mass Spectrometry. From 32 hours onwards the enrichment of {sup 68}Zn and {sup 70}Zn in urine declined proportionately so that FA could be determined according to the formula: FA - Enrichment (Oral/IV) {times} Dose (IV/Oral). FA was also determined from total cumulative fecal excretion of the oral isotopes. FA determinations from urine/feces for each of the four subjects (ZK{sup 1}-ZK{sup 4}) were: ZK1-.79 {plus minus} 03/.70 {plus minus} .01; ZK2 .79 {plus minus} .05/.69 {plus minus} .02; ZK3-.25 {plus minus} .01/.25 {plus minus} .01; and ZK4-.39 {plus minus} .02/.37 {plus minus}.02. Subjects ZK1 and ZK2 received the oral isotope without food while ZK3 and ZK4 received their oral isotope with meals. The authors conclude that FA of zinc can be determined by measurement of isotope enrichment in urine.

• OSTI ID: 5146503
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ZINC
METABOLISM
INTRAVENOUS INJECTION
MASS SPECTROSCOPY
ORAL ADMINISTRATION
TRACER TECHNIQUES
URINE
ZINC 68
ZINC 70
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
ELEMENTS
EVEN-EVEN NUCLEI
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
METALS
NUCLEI
SPECTROSCOPY
STABLE ISOTOPES
WASTES
ZINC ISOTOPES
560300* - Chemicals Metabolism & Toxicology