Transplantability of human lymphoid cell line, lymphoma, and leukemia in splenectomized and/or irradiated nude mice
The effects of splenectomy and/or whole-body irradiation of nude mice before xenotransplantation of lymphoid cell lines, lymphoma, and leukemia were studied. Transplantation after whole-body irradiation resulted in the increased ''take'' rate of three cultured cell lines (two of T-cell-derived acute lymphocytic leukemia and one of B-cell derived acute lymphocytic leukemia) and in the tumorous growth of Burkitt-derived Raji and spontaneously transformed lymphoblastoid cell lines. With splenectomy plus irradiation as a pretreatment, tumorous growth occurred in four other cell lines which were not transplantable after irradiation only (two cell lines of Epstein-Barr virus-transformed cord blood cells and one each of null acute lymphocytic leukemia and nodular lymphoma-derived cell lines). Direct transplantation of leukemia and lymphoma cells into the pretreated mice was successful in 7 of 24 cases (29%). B-cell-derived diffuse large lymphoid lymphoma was transplantable in three of seven cases (43%). However, lymphoma and leukemia of peripheral T-cell origin was difficult to transplant even with pretreatment, and only one pleomorphic T-cell lymphoma grew to a significant size (2 cm). One tumor each of B-cell-derived diffuse large lymphoid and T-cell diffuse lymphoblastic lymphoma became transplantable.
- Research Organization:
- National Cancer Center Research Inst., Tokyo, Japan
- OSTI ID:
- 5389275
- Journal Information:
- Cancer Res.; (United States), Vol. 40:7
- Country of Publication:
- United States
- Language:
- English
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IMMUNE REACTIONS
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SPLENECTOMY
TRANSPLANTS
WHOLE-BODY IRRADIATION
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560121* - Radiation Effects on Cells- External Source- (-1987)
560152 - Radiation Effects on Animals- Animals