UV-endonuclease from calf thymus with specificity toward pyrimidine dimers in DNA
- Tel-Aviv Univ., Israel
We describe the partial purification of an endonuclease from calf thymus that nicks phage PM2 DNA irradiated with uv doses producing only a few pyrimidine dimers per molecule. It has much less activity on DNA that has been subjected to enzymatic photoreactivation after uv irradiation. The calf thymus endonuclease is different from other mammalian uv-endonucleases so far described in that it seems to be dimer specific. The enzyme is stimulated by Mg/sup 2 +/ and is inactive in the presence of EDTA. It binds to uv-irradiated DNA-Sepharose from which it is released by low concentrations of KCl. Gel filtration data indicate that the endonuclease may belong to a high molecular weight protein or protein complex. The enzyme is very labile and freezing increases its lability.
- OSTI ID:
- 5371594
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 76:8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DIMERS
BIOLOGICAL REPAIR
DNA
BIOLOGICAL RADIATION EFFECTS
NUCLEASES
RADIOSENSITIVITY EFFECTS
EDTA
MOLECULAR WEIGHT
PYRIMIDINES
RADIOSENSITIVITY
RESPONSE MODIFYING FACTORS
THYMUS
ULTRAVIOLET RADIATION
AMINO ACIDS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BODY
CARBOXYLIC ACIDS
CHELATING AGENTS
ELECTROMAGNETIC RADIATION
ENZYMES
HETEROCYCLIC COMPOUNDS
LYMPHATIC SYSTEM
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHOTRANSFERASES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
TRANSFERASES
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
550201 - Biochemistry- Tracer Techniques