Ultra violet-endonuclease from calf thymus with specificity toward pyrimidine dimers in DNA

Journal Article · Wed Aug 01 00:00:00 EDT 1979 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.76.8.3746· OSTI ID:5061968

Waldstein, E A ^[1]; Peller, S; Setlow, R B

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1. Tel-Aviv Univ., Israel

We describe the partial purification of an endonuclease from calf thymus that nicks phage PM2 DNA irradiated with uv doses producing only a few pyrimidine dimers per molecule. It has much less activity on DNA that has been subjected to enzymatic photoreactivation after uv irradiation. The calf thymus endonuclease is different from other mammalian uv-endonucleases so far described in that it seems to be dimer specific. The enzyme is stimulated by Mg/sup 2 +/ and is inactive in the presence of EDTA. It binds to uv-irradiated DNA-Sepharose from which it is released by low concentrations of KCl. Gel filtration data indicate that the endonuclease may belong to a high molecular weight protein or protein complex. The enzyme is very labile and freezing increases it lability.

• OSTI ID: 5061968
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 76:8
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
DNA
BIOLOGICAL RADIATION EFFECTS
NUCLEASES
BIOLOGICAL REPAIR
BIOCHEMICAL REACTION KINETICS
CATTLE
DIMERS
EDTA
ENZYME ACTIVITY
MAGNESIUM IONS
MOLECULAR WEIGHT
PURIFICATION
PYRIMIDINES
SPECIFICITY
THYMUS
ULTRAVIOLET RADIATION
AMINO ACIDS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BODY
CARBOXYLIC ACIDS
CHARGED PARTICLES
CHELATING AGENTS
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
ENZYMES
HETEROCYCLIC COMPOUNDS
IONS
KINETICS
LYMPHATIC SYSTEM
MAMMALS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHOTRANSFERASES
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
RUMINANTS
TRANSFERASES
VERTEBRATES
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
550200 - Biochemistry