Specific changes in rapidly transported proteins during regeneration of the goldfish optic nerve
Double labeling methods were used to identify changes in the complement of proteins synthesized in the retinal ganglion cells and transported down the optic nerve during the process of axonal regeneration. Eight to 62 days after goldfish underwent a unilateral optic nerve crush, one eye was labeled with (3H)-, the other with (14C)proline. Control and regenerating optic nerves were dissected out and homogenized together after 5 hr, a time which allowed us to examine selectively membrane-bound components which migrate in the rapid phase of axoplasmic transport. Proteins from the two sides were so-purified and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Analysis of the 3H and 14C incorporation patterns along the gels revealed a radical shift away from the normal labeling spectrum during regeneration, with selective changes in labeling at particular molecular weights varying over a 3-fold range. Eight days after crushing the optic nerve, the greatest increases in labeling were seen for material with apparent molecular weights of 24,000 to 27,000, 44,000, and 210,000 daltons. These peaks declined thereafter, and on days 29 to 39, the most prominent increases were at 110,000 to 140,000 daltons. These studies indicate a continuously changing pattern in the synthesis and/or degradation of proteins that are rapidly transported down the optic nerve during regeneration and point to molecular species potential significance in the establishment of the visual map upon the brain.
- OSTI ID:
- 5104734
- Journal Information:
- J. Neurosci.; (United States), Vol. 1:3
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
ISOTOPE APPLICATIONS
NERVES
BIOLOGICAL RECOVERY
PROTEINS
BIOSYNTHESIS
TRITIUM COMPOUNDS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
GOLDFISH
MOLECULAR WEIGHT
NERVE CELLS
PROLINE
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AZOLES
CARBOXYLIC ACIDS
FISHES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
KINETICS
LABELLED COMPOUNDS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRROLES
PYRROLIDINES
REACTION KINETICS
RECOVERY
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques