Evidence that multiple species of aminoacylated transfer RNA are present in regenerating optic axons of goldfish

Journal Article · Wed Feb 01 00:00:00 EST 1984 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00964173· OSTI ID:6350153

Zanakis, M F; Eskin, B; Ingoglia, N A

This study reports that 4S RNA present in regenerating optic axons of goldfish is likely to be transfer RNA. Evidence is also presented which indicates that this transfer RNA is similar to transfer RNA found in tectal cells and that its aminoacylation is likely to occur both in retinal ganglion cells prior to axonal transport as well as in the axon itself. Fish with regenerating optic nerves received intraocular injections of (/sup 3/H)uridine followed 4 days later by intracranial injections of (/sup 14/C)uridine. Radioactive tectal 4S RNA was isolated 6 days after (/sup 3/H)uridine injections and chromatographed by BD cellulose chromatography. Optical density as well as radioactivity profiles for both (/sup 14/C)4S RNA (from tectal cells) and (/sup 3/H)4S RNA (90% of which originated from regenerating optic axons) were found to be similar to E. coli transfer RNA optical density profiles, indicating that the intra-axonal 4S RNA is likely to be transfer RNA. Moreover, comparisons of /sup 3/H//sup 14/C suggest that intra-axonal and cellular 4S RNAs are composed of similar species of transfer RNA. Results of other experiments indicate that aminoacylation of axonally transported tRNA occurs both in the retina and in optic axons subsequent to axonal transport.

• Research Organization: UMDNJ-New Jersey Medical School, Newark
• OSTI ID: 6350153
• Journal Information: Neurochem. Res.; (United States), Vol. 9:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
TRANSFER RNA
ACYLATION
TRITIUM COMPOUNDS
URIDINE
AMINO ACIDS
GOLDFISH
NERVE CELLS
RETINA
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AZINES
BODY
BODY AREAS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
EYES
FACE
FISHES
HEAD
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RIBOSIDES
RNA
SENSE ORGANS
SOMATIC CELLS
URACILS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques