Micrococcus luteus correndonucleases. III. Evidence for involvement in repair in vivo of two endonucleases specific for DNA containing pyrimidine dimers. [Micrococcus lutea]
- Johns Hopkins Univ., Baltimore
Involvement of Py--Py correndonucleases I and II in repair of ultraviolet radiation damage in vivo by Micrococcus luteus has been demonstrated by their absence in the ultraviolet-sensitive mutant DB-7 derived by treatment of the wild type parent with N-methyl-N'-nitro-N-nitrosoguanidine. The necessity for their combined action in DNA repair in M. luteus is shown by: (a) reactivation of ultraviolet-damaged phiX174 RFI DNA in incision-defective hosts after in vivo treatment with both enzymes, (b) correlation between survival after ultraviolet irradiation and the level of the two enzymes, and (c) increased levels of repair synthesis after ultraviolet irradiation of toluenized cells DB-400 with wild type correndonuclease levels when compared with the transformant DB-200 and the mutant DB-7, which lack one or both enzymes.
- OSTI ID:
- 5090056
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 252:18
- Country of Publication:
- United States
- Language:
- English
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Micrococcus luteus correndonucleases. II. Mechanism of action of two endonucleases specific for DNA containing pyrimidine dimers. [Micrococcus lutea; 15 J/m/sup 3/]
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Related Subjects
NUCLEASES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL REPAIR
MICROCOCCUS
MUTANTS
RADIATION INJURIES
ULTRAVIOLET RADIATION
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
ELECTROMAGNETIC RADIATION
ENZYMES
INJURIES
KINETICS
MICROORGANISMS
PHOSPHOTRANSFERASES
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
TRANSFERASES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)