Micrococcus luteus correndonucleases. II. Mechanism of action of two endonucleases specific for DNA containing pyrimidine dimers. [Micrococcus lutea; 15 J/m/sup 3/]

Journal Article · Sun Sep 25 00:00:00 EDT 1977 · J. Biol. Chem.; (United States)

OSTI ID:5038520

Riazuddin, S; Grossman, L

Py--Py correndonucleases I and II from Micrococcus luteus act exclusively on thymine-thymine, cytosine-cytosine, and thymine-cytosine cyclobutyl dimers in DNA, catalyzing incision 5' to the damage and generating 3'-hydroxyl and 5'-phosphoryl termini. Both enzymes initiate excision of pyrimidine dimers in vitro by correxonucleases and DNA polymerase I. The respective incised DNAs, however, differ in their ability to act as substrate for phage T4 polynucleotide ligase or bacterial alkaline phosphatase, suggesting that each endonuclease is specific for a conformationally unique site. The possibility that their respective action generates termini which represent different degrees of single strandedness is suggested by the unequal protection by Escherichia coli binding protein from the hydrolytic action of exonuclease VII.

• Research Organization: Johns Hopkins Univ., Baltimore
• OSTI ID: 5038520
• Journal Information: J. Biol. Chem.; (United States), Vol. 252:18
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
BIOLOGICAL RADIATION EFFECTS
MICROCOCCUS
ENZYMES
NUCLEASES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL REPAIR
CATALYSIS
DIMERS
PYRIMIDINES
ULTRAVIOLET RADIATION
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
KINETICS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHOTRANSFERASES
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
TRANSFERASES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)