Mammalian complex III heme dynamics studied with pump-probe spectroscopy and red light illuminations

O’Connor, Sean P.; Powell, Samantha M.; Rickman, John M.; Pope, Nathaniel J.; Noojin, Gary D.; Scully, Marlan O.; Denton, Michael L.; Yakovlev, Vladislav V.

doi:10.1364/boe.441118

Title: Mammalian complex III heme dynamics studied with pump-probe spectroscopy and red light illuminations

Journal Article · Fri Oct 22 00:00:00 EDT 2021 · Biomedical Optics Express

DOI:https://doi.org/10.1364/boe.441118· OSTI ID:1842787

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^[2]; Rickman, John M. ^[3]; Pope, Nathaniel J. ^[4]; Noojin, Gary D. ^[3]; Scully, Marlan O. ^[5];

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Texas A & M Univ., College Station, TX (United States)
National Research Council, Fort Sam Houston, TX (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Science Applications International Corporation, Fort Sam Houston, TX (United States)
Science Applications International Corporation, Fort Sam Houston, TX (United States); Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
Texas A & M Univ., College Station, TX (United States); Baylor Univ., Waco, TX (United States)
Air Force Research Lab. (AFRL), Fort Sam Houston, TX (United States)

The electronic or molecular mechanisms which initiate photobiomodulation (PBM) in cells are not yet fully understood. Porcine complex III (C-III) of the electron transport chain was characterized with transient absorption spectroscopy (TAS). Then we applied our recently developed continuous wave laser coupled TAS procedure (CW-TAS) to investigate the effect of red light irradiances on the heme dynamics of C-III in its c₁ reduced state. The time constants associated with the axial ligand photodissociation were found to be 3.3 ± 0.3 ps for the oxidized state and 4.9 ± 0.4 ps for the c₁ reduced state. The analysis of the CW-TAS procedure yielded no significant changes in the C-III heme dynamics. We rule out the possibility of 635 nm CW light at 4.7 mW/cm² inducing a PBM effect on the heme dynamic of C-III, specifically with the photodissociation of its axial ligand.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: Science Applications International Corporation (SAIC); Air Force Research Laboratory (AFRL); US Air Force Office of Scientific Research (AFOSR); US Department of the Navy, Office of Naval Research (ONR); National Science Foundation (NSF); Army Research Laboratory (ARL); National Institutes of Health (NIH); Cancer Prevention and Research Institute of Texas

Grant/Contract Number:: AC05-76RL01830; FA8650-14-D-6519; FA8650-19-C-6024; A-1261; FA9550-20-1-0366; N00014-20-1-2184; PHY-2013771; 19RHCOR067; DBI-1455671; ECCS-1509268; CMMI-1826078; FA9550-15-1-0517; FA9550-18-1-0141; W911NF-17-2-0144; W81xWH2010777: N00014-16-1-2578; 1R01GM127696-01; 1R21GM142107: RP180588

OSTI ID:: 1842787

Report Number(s):: PNNL-SA-166055

Journal Information:: Biomedical Optics Express, Vol. 12, Issue 11; ISSN 2156-7085

Publisher:: Optical Society of America (OSA)Copyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Title: Mammalian complex III heme dynamics studied with pump-probe spectroscopy and red light illuminations

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